Peczyk Klaudia, Siupka Piotr, Magurno Franco, Malicka Monika, Piotrowska-Seget Zofia
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia, Jagiellońska 28, Katowice, 40-032, Poland.
Mol Genet Genomics. 2025 Feb 22;300(1):24. doi: 10.1007/s00438-025-02232-y.
The study aimed to explore the genetic and functional potential of mycorrhizal helper bacteria (MHB) strains isolated from polluted soil, focusing on their ability to enhance plant growth and ameliorate the adverse effects of polycyclic aromatic hydrocarbons (PAHs). We sequenced the genomes of three MHB strains isolated from soil contaminated with PAHs and phenol. Moreover, experiments were carried out to check if these bacteria have ability to stimulate the growth of arbuscular mycorrhizal fungi (AMF) and promote plant development. Phylogenomic analysis identified the strains as belonging to the Streptomyces, Pantoea, and Bacillus genera, all exhibiting high tolerance to hydrocarbons. Genome mining revealed genes encoding enzymes for the degradation of aromatic compounds, alongside biosynthetic gene clusters for secondary metabolites such as siderophores and antibiotics. Laboratory experiments confirmed that the studied MHB strains enhance AMF development and spore production while exhibiting plant growth-promoting mechanisms such as siderophore and ammonia production, phosphate solubilization, and cellulolytic enzyme synthesis. These findings highlight the potential application of MHB in microbial-assisted remediation of hydrocarbon-contaminated soils through the tripartite plant-MHB-AMF system.
该研究旨在探索从污染土壤中分离出的菌根辅助细菌（MHB）菌株的遗传和功能潜力，重点关注其促进植物生长和改善多环芳烃（PAHs）不利影响的能力。我们对从受PAHs和苯酚污染的土壤中分离出的三株MHB菌株的基因组进行了测序。此外，还进行了实验以检查这些细菌是否有能力刺激丛枝菌根真菌（AMF）的生长并促进植物发育。系统基因组分析确定这些菌株属于链霉菌属、泛菌属和芽孢杆菌属，均表现出对碳氢化合物的高耐受性。基因组挖掘揭示了编码芳香化合物降解酶的基因，以及铁载体和抗生素等次生代谢物的生物合成基因簇。实验室实验证实，所研究的MHB菌株可促进AMF的发育和孢子产生，同时表现出促进植物生长的机制，如铁载体和氨的产生、磷的溶解以及纤维素酶的合成。这些发现突出了MHB通过植物 - MHB - AMF三方系统在微生物辅助修复碳氢化合物污染土壤中的潜在应用。
