de Oliveira Sales-Junior Romulo, de Moura Pereira Bharbara, Ricci Rafaela, da Silva Machado Nathália Evelyn, Alvarado Julissa Denisse Arguello, Carreto Ana Beatriz, Ervolino Edilson, Kishen Anil, Cintra Luciano Tavares Ângelo, Gomes-Filho João Eduardo
Department of Preventive and Restorative Dentistry. São Paulo State University (Unesp), School of Dentistry, Araçatuba, Brazil.
Department of Basic Sciences, São Paulo State University (Unesp). School of Dentistry, Araçatuba, Brazil.
Arch Oral Biol. 2025 May;173:106206. doi: 10.1016/j.archoralbio.2025.106206. Epub 2025 Feb 18.
To analyze the effect of supplementation with dealcoholized red wine on the severity of apical periodontitis after it had been established.
Thirty-two male Wistar rats were arranged into four groups: water as control, dealcoholized red wine, red wine, and alcohol. Apical periodontitis was induced by pulp exposure for 30 days when supplementation started and continued daily for 30 days. After the supplementation period, the rats were euthanized, and maxillae and jaws were removed to evaluate the inflammatory response and the bone resorption through microtomographic, histological and immunohistochemical analysis. Statistical tests were applied at 5 % significance.
The dealcoholized red wine group demonstrated a significant reduction in bone resorption volume and increase in bone volume fraction and trabecular parameters (p < 0.05). The dealcoholized red wine and red wine groups exhibited mild inflammation compared to the control and alcohol groups that showed moderate and severe inflammation respectively (p < 0.05). The immunoreaction was significantly lower in the dealcoholized red wine group than in the control and alcohol groups for the TNF-α, IL-1β and TRAP cells (p < 0.05). Furthermore, OPG in dealcoholized red wine was similar to red wine and control but higher than in the alcohol group (p < 0.05).
Supplementation with dealcoholized red wine in rats decreased inflammation and bone loss in established apical periodontitis. This study highlights the potential of systemic administration of polyphenols from red wine in decreasing inflammation and bone resorption of established apical periodontitis in rats.
分析补充脱醇红酒对已形成的根尖周炎严重程度的影响。
将32只雄性Wistar大鼠分为四组：水作为对照组、脱醇红酒组、红酒组和酒精组。通过暴露牙髓诱导根尖周炎30天，从开始补充时起持续30天，每天补充。补充期结束后，对大鼠实施安乐死，取出上颌骨和下颌骨，通过显微断层扫描、组织学和免疫组织化学分析评估炎症反应和骨吸收情况。采用显著性水平为5%的统计学检验。
脱醇红酒组的骨吸收体积显著减少，骨体积分数和小梁参数增加（p<0.05）。与分别表现为中度和重度炎症的对照组和酒精组相比，脱醇红酒组和红酒组表现出轻度炎症（p<0.05）。脱醇红酒组中TNF-α、IL-1β和TRAP细胞的免疫反应明显低于对照组和酒精组（p<0.05）。此外，脱醇红酒中的骨保护素与红酒和对照组相似，但高于酒精组（p<0.05）。
给大鼠补充脱醇红酒可减轻已形成的根尖周炎的炎症和骨质流失。本研究强调了系统性给予红酒中的多酚在减轻大鼠已形成的根尖周炎的炎症和骨吸收方面的潜力。
