Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil.
Department of Cariology, Restorative Sciences and Endodontics, University of Michigan, Ann Arbor, Michigan, USA.
Int Endod J. 2021 Dec;54(12):2276-2289. doi: 10.1111/iej.13633. Epub 2021 Oct 4.
To evaluate the effect of red wine consumption or its polyphenols on the inflammation/resorption processes associated with apical periodontitis in rats.
Thirty-two three-month-old Wistar rats had apical periodontitis induced in four first molars and were then arranged into four groups: control (C)-rats with apical periodontitis; wine (W)-rats with apical periodontitis receiving 4.28 ml/kg of red wine; resveratrol+quercetin (R+Q)-rats with apical periodontitis receiving 4.28 ml/kg of a solution containing 1.00 mg/L of quercetin and 0.86 mg/L of resveratrol and alcohol (ALC)-rats with apical periodontitis receiving the alcoholic dose contained in the wine. The oral gavage treatments were administered daily, from day 0 to day 45. On the 15th day, apical periodontitis was induced, and on the 45th day, the animals were euthanized. Histological, immunohistochemical (RANKL, OPG, TRAP, IL-10, TNF-⍺ and IL-1β) and micro-computed tomography for bone resorption analysis were performed in the jaws. The Kruskal-Wallis with Dunn's test was performed for nonparametric data, and the anova with Tukey's test for parametric data, p < .05.
The median score of the inflammatory process was significantly lower in the R+Q group (1) compared to the C (2) (p = .0305) and ALC (3) (p = .0003) groups, and not different from the W (1.5) group. The immunolabeling for OPG was significantly higher in the R+Q group (p = .0054) compared to all groups; the same was observed for IL-10 (p = .0185), different from groups C and ALC. The R+Q group had the lowest TRAP cell count (p < .0001), followed by the W group, both inferior to C and ALC groups. The lowest bone resorption value was in the R+Q group (0.50mm ± 0.21mm ), significantly lower (p = .0292) than the C group (0.88mm ± 0.10mm ). The W group (0.60 mm ± 0.25 mm ) and R+Q group had less bone resorption compared to the ALC group (0.97 mm ± 0.22 mm ), p = .0297 and p = .0042, respectively.
Red wine administration to rats for 15 days before induction of apical periodontitis decreased inflammation, TRAP marking and periapical bone resorption compared to alcohol. Resveratrol-quercetin administration reduced the inflammatory process in apical periodontitis, periapical bone resorption, and altered the OPG, IL-10 and TRAP expression compared to C and ALC groups.
评估红酒消费或其多酚对大鼠根尖周炎相关炎症/吸收过程的影响。
32 只 3 月龄 Wistar 大鼠在 4 颗第一磨牙中诱导根尖周炎，然后分为 4 组：对照（C）-患有根尖周炎的大鼠；酒（W）-患有根尖周炎的大鼠接受 4.28ml/kg 的红酒；白藜芦醇+槲皮素（R+Q）-患有根尖周炎的大鼠接受含有 1.00mg/L 槲皮素和 0.86mg/L 白藜芦醇的溶液 4.28ml/kg 和酒精（ALC）-患有根尖周炎的大鼠接受红酒中含有的酒精剂量。口服灌胃治疗从第 0 天到第 45 天每天进行。第 15 天诱导根尖周炎，第 45 天处死动物。对颌骨进行组织学、免疫组织化学（RANKL、OPG、TRAP、IL-10、TNF-α 和 IL-1β）和骨吸收分析的微计算机断层扫描。非参数数据采用 Kruskal-Wallis 检验与 Dunn's 检验，参数数据采用方差分析与 Tukey 检验，p<0.05。
与 C（2）组（p=0.0305）和 ALC（3）组（p=0.0003）相比，R+Q 组（1）的炎症过程中位数评分显著降低（1），与 W（1.5）组无差异。与所有组相比，R+Q 组的 OPG 免疫标记明显更高（p=0.0054）；IL-10 也是如此（p=0.0185），与 C 和 ALC 组不同。R+Q 组的 TRAP 细胞计数最低（p<0.0001），其次是 W 组，均低于 C 和 ALC 组。R+Q 组的骨吸收值最低（0.50mm±0.21mm），显著低于 C 组（0.88mm±0.10mm）（p=0.0292）。与 ALC 组（0.97mm±0.22mm）相比，W 组（0.60mm±0.25mm）和 R+Q 组的骨吸收较少，p=0.0297 和 p=0.0042。
在诱导根尖周炎前 15 天给大鼠灌酒可降低炎症、TRAP 标记和根尖周骨吸收，与酒精相比。与 C 和 ALC 组相比，白藜芦醇-槲皮素给药减少了根尖周炎、根尖周骨吸收，并改变了 OPG、IL-10 和 TRAP 的表达。
