de Moura João Daniel Mendonça, Santos Vinicius Ruan Neves Dos, Bittencourt Leonardo Oliveira, Collares Fabrício Mezzomo, Mendes Paulo Fernando Santos, Matos-Sousa José Mario, Risuenho Penaido Beatriz Rodrigues, Perdigão José Messias, Rogez Herve, Lima Rafael Rodrigues, Rodrigues Patricia de Almeida
Department of Dentistry, School of Dentistry, Federal University of Pará, Belém, Pará, Brazil.
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, Brazil.
Int Endod J. 2025 Aug;58(8):1211-1227. doi: 10.1111/iej.14247. Epub 2025 May 8.
This study aimed to investigate the effects of açai on bone and systemic damage caused by apical periodontitis (AP) in an animal model.
In this experimental study, 32 male Wistar rats were randomly allocated into six groups-Control 14 days (n = 4), Control 28 days (n = 4), AP 14 days (n = 4), AP 28 days (n = 4), AP plus açai treatment for 14 days (n = 8) and AP plus açai treatment for 28 days (n = 8). Apical periodontitis was induced under general anaesthesia by exposing the pulp of the first molars to the oral environment. Daily treatments were administered by gavage at a dose of 0.01 mL/g, using either saline solution or clarified açai. At the end of each experimental period, periapical lesions were quantitatively evaluated by micro-computed tomography (micro-CT) and histopathological analyses, whilst systemic oxidative stress was assessed through biochemical assays. Data normality was verified using the Shapiro-Wilk test, followed by one-way anova and Tukey's post hoc test (p < .05).
Micro-CT analysis revealed that açai reduced apical periodontitis lesion volume and improved bone quality (p < .05). Histopathological evaluation corroborated these findings, revealing moderate inflammation at 14 days and more pronounced, heterogeneous inflammatory responses at 28 days, with no significant differences between groups. Additionally, açai modulated systemic oxidative biochemistry, enhanced antioxidant defences and reduced pro-oxidant damage after 28 days.
Oral açai administration was associated with reduced progression of apical periodontitis and improved bone quality, suggesting its potential as a protective antioxidant in endodontic treatment, minimizing both local and systemic damage.
本研究旨在探讨阿萨伊果对动物模型中根尖周炎（AP）所致骨及全身损伤的影响。
在本实验研究中，32只雄性Wistar大鼠被随机分为六组——14天对照组（n = 4）、28天对照组（n = 4）、14天AP组（n = 4）、28天AP组（n = 4）、AP加阿萨伊果治疗14天组（n = 8）和AP加阿萨伊果治疗28天组（n = 8）。在全身麻醉下，通过将第一磨牙的牙髓暴露于口腔环境来诱导根尖周炎。每日通过灌胃给予剂量为0.01 mL/g的生理盐水或澄清的阿萨伊果进行治疗。在每个实验周期结束时，通过显微计算机断层扫描（micro-CT）和组织病理学分析对根尖周病变进行定量评估，同时通过生化检测评估全身氧化应激。使用Shapiro-Wilk检验验证数据正态性，随后进行单因素方差分析和Tukey事后检验（p < 0.05）。
Micro-CT分析显示，阿萨伊果减少了根尖周炎病变体积并改善了骨质量（p < 0.05）。组织病理学评估证实了这些发现，显示14天时炎症为中度，28天时炎症反应更明显且异质性更强，各组之间无显著差异。此外，阿萨伊果调节了全身氧化生物化学，增强了抗氧化防御并在28天后减少了促氧化损伤。
口服阿萨伊果与根尖周炎进展减缓及骨质量改善相关，表明其在牙髓治疗中作为保护性抗氧化剂的潜力，可将局部和全身损伤降至最低。
