Nouwen E J, Kühn E R
J Endocrinol. 1985 Jun;105(3):371-7. doi: 10.1677/joe.0.1050371.
Adult male frogs (Rana ridibunda) were subjected to several volumetric and osmometric stimuli and the influence on circulating concentrations of arginine vasotocin (AVT) and mesotocin was studied by the use of highly specific radioimmunoassays. During progressive blood withdrawal (haemorrhage group) urine flow decreased to zero, whereas no change occurred in the plasma and urine osmolality. Control levels of 34.3 +/- 7.3 pmol AVT/1 gradually increased up to 638.3 +/- 179.1 pmol/1 (P less than 0.001) after a blood loss of up to 50-60% of the blood volume. Plasma mesotocin concentrations also increased from 42.4 +/- 9.2 to 70.8 +/- 12.0 pmol/1 (n = 7). Hypervolaemia, produced by the repeated intravenous injection of isotonic Ringer solution, increased the urine flow and osmolality compared to controls but had no influence on the plasma levels of AVT and mesotocin. Hypernatraemia without volume change profoundly increased the urine osmolality but the urine flow was not affected; the plasma concentrations of AVT and mesotocin remained at the control level. Finally, during a 1-h immobilization stress a pronounced antidiuresis occurred in the presence of a constant plasma and urine osmolality and control plasma levels of AVT and mesotocin. It is concluded that the release of AVT and, to a smaller extent, of mesotocin is under volumetric control.
对成年雄性池蛙（Rana ridibunda）施加了多种容量和渗透压刺激，并通过使用高度特异性的放射免疫测定法研究了其对精氨酸加压素（AVT）和中催产素循环浓度的影响。在渐进性失血过程中（出血组），尿流量降至零，而血浆和尿渗透压没有变化。在失血高达血容量的50 - 60%后，AVT的对照水平从34.3±7.3 pmol/1逐渐升高至638.3±179.1 pmol/1（P < 0.001）。血浆中催产素浓度也从42.4±9.2升高至70.8±12.0 pmol/1（n = 7）。通过反复静脉注射等渗林格溶液产生的血容量过多，与对照组相比增加了尿流量和渗透压，但对AVT和中催产素的血浆水平没有影响。无容量变化的高钠血症显著增加了尿渗透压，但尿流量未受影响；AVT和中催产素的血浆浓度保持在对照水平。最后，在1小时的固定应激期间，在血浆和尿渗透压恒定以及AVT和中催产素血浆水平处于对照的情况下，出现了明显的抗利尿作用。得出的结论是，AVT以及在较小程度上中催产素的释放受容量控制。
