Heido John, Keng Simon, Poyrazoglu Hulya, Priyam Ekta, Jayasinghe Sajith
Chemistry and Biochemistry, California State University San Marcos, San Marcos, California, United States.
MicroPubl Biol. 2025 Feb 7;2025. doi: 10.17912/micropub.biology.001394. eCollection 2025.
The discovery of Wza, an octomeric helical barrel integral bacterial outer-transmembrane protein, has challenged the widely held understanding that all integral outer-membrane proteins of Gram-negative bacteria are closed β-barrels composed of transmembrane β- strands. Wza is a member of the Outer-Membrane Polysaccharide Exporter family and our bioinformatics analysis suggests that other members of the family may also contain outer-membrane transmembrane segments that are helical. A review of the literature indicates that in addition to Wza, outer-membrane core complex proteins of the type IV secretion systems also contain transmembrane segments that are helical.
八聚体螺旋桶状细菌外膜整合蛋白Wza的发现，对一种广泛持有的观点提出了挑战，即革兰氏阴性菌的所有外膜整合蛋白都是由跨膜β链组成的封闭β桶。Wza是外膜多糖转运蛋白家族的成员，我们的生物信息学分析表明，该家族的其他成员可能也含有螺旋状的外膜跨膜片段。文献综述表明，除Wza外，IV型分泌系统的外膜核心复合体蛋白也含有螺旋状的跨膜片段。
