Li Qiuye, Zhang Li, Xu Qin, Zhang Peng, Zhu Shiwei
Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
Department of Neurosciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
bioRxiv. 2025 Feb 16:2025.02.14.638381. doi: 10.1101/2025.02.14.638381.
cryo-electron tomography (cryo-ET) has recently been widely used in observing subcellular structures and macromolecules in their native states at high resolution. One of the reasons that it has not been more widely adopted by cell biologists and structural biologists is the difficulties in sample preparation. Here we present the Sandwich-LIke Culturing Kit (SLICK), simplifying the procedure and increasing the throughput for sample preparation for cryo-ET.
冷冻电子断层扫描（cryo-ET）最近已被广泛用于高分辨率观察天然状态下的亚细胞结构和大分子。它尚未被细胞生物学家和结构生物学家更广泛采用的原因之一是样品制备存在困难。在此，我们展示了类似三明治培养试剂盒（SLICK），它简化了流程并提高了用于cryo-ET的样品制备通量。
