• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

New insights into the molecular architecture of neurons by cryo-electron tomography.

作者信息

Petrovic Arsen, Do Thanh Thao, Fernández-Busnadiego Rubén

机构信息

University Medical Center Göttingen, Institute for Neuropathology, Göttingen, 37077, Germany; Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen, Göttingen, 37077, Germany.

University Medical Center Göttingen, Institute for Neuropathology, Göttingen, 37077, Germany.

出版信息

Curr Opin Neurobiol. 2025 Feb;90:102939. doi: 10.1016/j.conb.2024.102939. Epub 2024 Dec 11.

DOI:10.1016/j.conb.2024.102939
PMID:39667254
Abstract

Cryo-electron tomography (cryo-ET) visualizes natively preserved cellular ultrastructure at molecular resolution. Recent developments in sample preparation workflows and image processing tools enable growing applications of cryo-ET in cellular neurobiology. As such, cryo-ET is beginning to unravel the in situ macromolecular organization of neurons using samples of increasing complexity. Here, we highlight advances in cryo-ET technology and review its recent use to study neuronal architecture and its alterations under disease conditions.

摘要

相似文献

1
New insights into the molecular architecture of neurons by cryo-electron tomography.
Curr Opin Neurobiol. 2025 Feb;90:102939. doi: 10.1016/j.conb.2024.102939. Epub 2024 Dec 11.
2
Recent technical advances in cellular cryo-electron tomography.细胞冷冻电子断层扫描技术的最新进展。
Int J Biochem Cell Biol. 2024 Oct;175:106648. doi: 10.1016/j.biocel.2024.106648. Epub 2024 Aug 23.
3
Insights into the molecular organization of the neuron by cryo-electron tomography.通过冷冻电子断层扫描对神经元分子组织的深入了解。
J Electron Microsc (Tokyo). 2011;60 Suppl 1:S137-48. doi: 10.1093/jmicro/dfr018.
4
Handling Difficult Cryo-ET Samples: A Study with Primary Neurons from Drosophila melanogaster.处理困难的冷冻电镜样品：使用黑腹果蝇原代神经元进行的研究。
Microsc Microanal. 2023 Dec 21;29(6):2127-2148. doi: 10.1093/micmic/ozad125.
5
Developments in cryo-electron tomography for in situ structural analysis.用于原位结构分析的冷冻电子断层扫描技术的发展
Arch Biochem Biophys. 2015 Sep 1;581:78-85. doi: 10.1016/j.abb.2015.04.006. Epub 2015 Apr 25.
6
Capturing actin assemblies in cells using in situ cryo-electron tomography.使用原位冷冻电子断层成像术捕获细胞中的肌动蛋白组装。
Eur J Cell Biol. 2022 Jun-Aug;101(3):151224. doi: 10.1016/j.ejcb.2022.151224. Epub 2022 Apr 9.
7
Cryo-electron tomography: methodology, developments and biological applications.低温电子断层扫描技术：方法学、进展和生物学应用。
J Microsc. 2011 Jun;242(3):221-7. doi: 10.1111/j.1365-2818.2010.03478.x. Epub 2010 Dec 23.
8
Biological Applications at the Cutting Edge of Cryo-Electron Microscopy.冷冻电子显微镜前沿的生物学应用
Microsc Microanal. 2018 Aug;24(4):406-419. doi: 10.1017/S1431927618012382.
9
The promise and the challenges of cryo-electron tomography.冷冻电镜断层成像的前景与挑战。
FEBS Lett. 2020 Oct;594(20):3243-3261. doi: 10.1002/1873-3468.13948. Epub 2020 Oct 23.
10
Recent advances and current trends in cryo-electron microscopy.冷冻电子显微镜技术的最新进展和当前趋势。
Curr Opin Struct Biol. 2022 Dec;77:102484. doi: 10.1016/j.sbi.2022.102484. Epub 2022 Oct 28.

引用本文的文献

1
SLICK: A Sandwich-LIke Culturing Kit for Cryo-ET Sample Preparation.SLICK：一种用于冷冻电子断层扫描样品制备的类似三明治的培养试剂盒。
bioRxiv. 2025 Feb 16:2025.02.14.638381. doi: 10.1101/2025.02.14.638381.