De La Reé-Rodríguez Sandra Carolina, González María Jesús, Fernández Ingrid, Garrido José Luis, Silva-Campa Erika, Parra-Vergara Norma Violeta, López-Saiz Carmen María, Medina Isabel
Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Hermosillo 83000, Sonora, Mexico.
Química de Productos Marinos, Instituto de Investigaciones Marinas, 36208 Vigo, Pontevedra, Spain.
Mar Drugs. 2025 Jan 27;23(2):59. doi: 10.3390/md23020059.
Marine organisms are a vital source of biologically active compounds. Organic extracts from the muscle of the Pacific white shrimp () have shown antiproliferative effects on tumor cells, including breast adenocarcinoma. This study aimed to analyze these extracts' composition and confirm their specificity for breast adenocarcinoma cells without harming normal cells. An organic chloroform extract from muscle was divided using a solvent partition procedure with methanol and hexane. The methanolic partition was fractionated through an open preparative liquid chromatography column to isolate compounds with biological activity, that were later tested on MDA-MB-231 (breast adenocarcinoma), and recently tested on MCF10-A (non-cancerous breast epithelial cells). Cells incubated with these fractions were assessed for viability and morphological changes using fluorescence confocal microscopy. Fractions F#13 and F#14 reduced MDA-MB-231 cancer cell viability at 100 µg/mL without affecting non-cancerous MCF-10A cells, inducing apoptosis-related changes in cancer cells. These fractions contained EPA and DHA free fatty acids, specifically F#13 contained free and esterified astaxanthin as well. The high levels of free linoleic acid 18:2 ω-6, EPA, and DHA (in a 2:1 ratio, EPA:DHA), along with free and esterified astaxanthin in F#13, significantly reduced breast adenocarcinoma cell viability, nearly to that achieved by cisplatin, a chemotherapy drug.
海洋生物是生物活性化合物的重要来源。太平洋白虾（）肌肉的有机提取物已显示出对肿瘤细胞，包括乳腺腺癌的抗增殖作用。本研究旨在分析这些提取物的成分，并确认它们对乳腺腺癌细胞的特异性，同时不损害正常细胞。采用甲醇和己烷的溶剂分配程序对来自肌肉的有机氯仿提取物进行分离。甲醇分配物通过开放式制备液相色谱柱进行分馏，以分离具有生物活性的化合物，随后在MDA-MB-231（乳腺腺癌）上进行测试，最近又在MCF10-A（非癌性乳腺上皮细胞）上进行测试。使用荧光共聚焦显微镜评估与这些组分孵育的细胞的活力和形态变化。F#13和F#14组分在100μg/mL时降低了MDA-MB-231癌细胞的活力，而不影响非癌性MCF-10A细胞，诱导癌细胞发生凋亡相关变化。这些组分含有EPA和DHA游离脂肪酸，具体而言，F#13还含有游离和酯化的虾青素。F#13中高水平的游离亚油酸18:2ω-6、EPA和DHA（EPA:DHA比例为2:1），以及游离和酯化的虾青素，显著降低了乳腺腺癌细胞的活力，几乎达到了化疗药物顺铂的效果。
