采用放射免疫分析法测定人血浆中5α-雄烷-3α,17β-二醇和5α-雄烷-3β,17β-二醇的含量。
Determination of 5 alpha-androstane-3 alpha, 17 beta-diol and 5 alpha-androstane-3 beta,17 beta-diol in human plasma by radioimmunoassay.
作者信息
Jacolot F, Adeline J, Berthou F, Floch H H
出版信息
J Steroid Biochem. 1985 Apr;22(4):533-7. doi: 10.1016/0022-4731(85)90174-8.
5 alpha-Androstane-3 alpha,17 beta-diol (3 alpha-diol) and 5 alpha-androstane-3 beta,17 beta-diol (3 beta-diol) were measured in human peripheral plasma by radioimmunoassay using celite microcolumn purification. The antisera used for the assay were obtained by immunization of rabbits with 3 alpha,17 beta-dihydroxy-5 alpha-androstane-6-(O-carboxymethyl) oxime: BSA for 3 alpha-diol and 3 beta,17 beta-dihydroxy-5 alpha-androstane-15 alpha-carboxymethyl: BSA for 3 beta-diol. The concentrations (pg/ml +/- SD) of the two diols in normal male and female plasma are respectively: 216 +/- 51 and 49 +/- 32 for 3 alpha-diol, 239 +/- 76 and 82 +/- 45 for 3 beta-diol. Comparison of these results with published ones shows that 3 beta diol concentrations were significantly lower. The high specificity of the assay is due to chromatography on celite microcolumns, allowing elimination of 5-androstene-3 beta,17 beta-diol from the plasma sample.
采用硅藻土微柱纯化的放射免疫分析法测定人外周血血浆中的5α-雄甾烷-3α,17β-二醇（3α-二醇）和5α-雄甾烷-3β,17β-二醇（3β-二醇）。用于该测定的抗血清是通过用3α,17β-二羟基-5α-雄甾烷-6-（O-羧甲基）肟：牛血清白蛋白免疫兔获得3α-二醇的抗血清，以及用3β,17β-二羟基-5α-雄甾烷-15α-羧甲基：牛血清白蛋白免疫兔获得3β-二醇的抗血清。正常男性和女性血浆中两种二醇的浓度（pg/ml±标准差）分别为：3α-二醇为216±51和49±32，3β-二醇为239±76和82±45。将这些结果与已发表的结果进行比较表明，3β-二醇的浓度显著较低。该测定的高特异性归因于在硅藻土微柱上的色谱分析，使得能够从血浆样品中去除5-雄烯-3β,17β-二醇。
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