Laband P, Tresguerres J A, Lisboa B P, Volkwein U, Tamm J
Acta Endocrinol (Copenh). 1978 Aug;88(4):778-86.
Antibodies have been raised in rabbits against 3alpha, 17beta-dihydroxy-5alpha-androstane-6-0-carboxymethyloxime coupled with Cohn's fraction IV-4. The antiserum exhibited significant cross reactions with 5beta-androstane-3alph1, 17beta-diol, 5alpha-dihydrotestosterone, and testosterone. No cross reactions were observed with 5alpha-androstane-3beta,17beta-diol and 5-androstene-3beta,17beta-diol. The methodological criteria for the measurement of 5alpha-androstane-3alpha, 17beta-diol in human plasma were as follows: The specificity was ensured by separating the cross reacting steroids by thin layer chromatography. The intraassay and interassay coefficients of variation were found to be 6.2 and 10.2%, respectively. The sensitivity was 30 pg. The recovery of different amounts of 5alpha-androstane-3alpha,17beta-diol added to human plasma (80, 120, and 200 pg) yielded 91.3, 92.5, and 93.5%, respectively. The following concentrations of 5alpha-androstane-3alpha,17beta-diol have been determined in human plasma (mean +/- SD, ng/dl): Normal males: 18.98 +/- 5.9; normal females: 2.65 +/- 0.27; females with idiopathic hirsutism: 11.9 +/- 6.4; prepubertal children: not detectable.
已在兔体内制备了针对与科恩氏IV-4组分偶联的3α，17β-二羟基-5α-雄甾烷-6-O-羧甲基肟的抗体。抗血清与5β-雄甾烷-3α，17β-二醇、5α-双氢睾酮和睾酮表现出显著的交叉反应。未观察到与5α-雄甾烷-3β，17β-二醇和5-雄烯-3β，17β-二醇的交叉反应。测定人血浆中5α-雄甾烷-3α，17β-二醇的方法学标准如下：通过薄层色谱法分离交叉反应的甾体来确保特异性。测定内和测定间变异系数分别为6.2%和10.2%。灵敏度为30 pg。向人血浆中添加不同量的5α-雄甾烷-3α，17β-二醇（80、120和200 pg）后的回收率分别为91.3%、92.5%和93.5%。已测定人血浆中5α-雄甾烷-3α，17β-二醇的以下浓度（平均值±标准差，ng/dl）：正常男性：18.98±5.9；正常女性：2.65±0.27；特发性多毛症女性：11.9±6.4；青春期前儿童：未检测到。
