Costa Tainá de Lima, Puppin-Rontani Regina Maria, de Castilho Aline Rogéria Freire
Departamento de Ciências da Saúde e Odontologia Infantil, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, Piracicaba 13414-903, Brazil.
Departamento de Odontologia Restauradora, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, Piracicaba 13414-903, Brazil.
J Funct Biomater. 2025 Feb 18;16(2):70. doi: 10.3390/jfb16020070.
This study introduces innovative varnishes incorporating natural bioactive compounds to inhibit the formation of oral dual biofilms, a critical contributor to dental caries and other oral diseases. The purpose of this study was to evaluate the effectiveness of bioactive varnishes containing -farnesol, quercetin, and theobromine in inhibiting the formation of mixed and biofilms. Mixed biofilms of UA159 and SC5314 were grown in 96-well plates containing a specialized culture medium. Approximately 0.2 mL of experimental varnishes with A-1.5% or B-4.5% concentrations of -farnesol, quercetin, and theobromine were separately added to the wells using a disposable applicator, with a vehicle varnish (lacking bioactives) serving as the control. Biofilms were incubated at 37 °C with 5% CO for 24 h. Microbial viability was determined in terms of colony-forming units per milliliter (CFU/mL), and biofilm morphology was evaluated qualitatively via scanning electron microscopy (SEM). Statistical analyses were performed using ANOVA/Tukey tests at a 5% significance level. Varnishes A and B achieved significant reductions in microbial populations within the biofilms ( < 0.05) compared to the vehicle control (C). SEM imaging revealed marked structural disruptions in the biofilms, validating the quantitative results. Higher bioactive concentrations demonstrated enhanced inhibitory effects. Bioactive varnishes enriched with theobromine, quercetin, and -farnesol represent a novel and effective strategy for inhibiting oral dual biofilm development, offering a promising advancement in preventive dentistry.
本研究引入了含有天然生物活性化合物的创新型清漆，以抑制口腔双重生物膜的形成，口腔双重生物膜是龋齿和其他口腔疾病的关键促成因素。本研究的目的是评估含有金合欢醇、槲皮素和可可碱的生物活性清漆在抑制混合生物膜和白色念珠菌生物膜形成方面的有效性。在含有特殊培养基的96孔板中培养变形链球菌UA159和白色念珠菌SC5314的混合生物膜。使用一次性涂抹器将约0.2 mL浓度为A - 1.5%或B - 4.5%的含有金合欢醇、槲皮素和可可碱的实验清漆分别添加到孔中，以不含生物活性成分的载体清漆作为对照。生物膜在37°C、5%二氧化碳条件下孵育24小时。通过每毫升菌落形成单位（CFU/mL）确定微生物活力，并通过扫描电子显微镜（SEM）对生物膜形态进行定性评估。使用ANOVA/Tukey检验在5%的显著性水平上进行统计分析。与载体对照（C）相比，清漆A和清漆B使生物膜内的微生物数量显著减少（P < 0.05）。SEM成像显示生物膜结构明显破坏，证实了定量结果。较高的生物活性浓度显示出更强的抑制作用。富含可可碱、槲皮素和金合欢醇的生物活性清漆代表了一种抑制口腔双重生物膜形成的新颖有效策略，为预防牙科提供了有前景的进展。
