Dereventsova Alena V, Klimentov Alexander S, Kholodilov Ivan S, Belova Oxana A, Butenko Alexander M, Karganova Galina G
Laboratory of Biochemistry, Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS (Institute of Poliomyelitis), 108819 Moscow, Russia.
Laboratory of Biology of Arboviruses, Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS (Institute of Poliomyelitis), 108819 Moscow, Russia.
Methods Protoc. 2025 Feb 18;8(1):20. doi: 10.3390/mps8010020.
The family , part of the order , includes arboviruses and arthropod-associated viruses, with sandflies, mosquitoes, and ticks as primary vectors. Historically, only sandfly/mosquito-borne phenuiviruses were associated with human diseases, but the emergence of severe fever with thrombocytopenia syndrome (SFTS) has highlighted the potential of tick-borne phenuiviruses as human pathogens. Recent discoveries of new arthropod-associated viruses, some of which remain unclassified, underscore the need for sensitive detection and differentiation methods, particularly in regions where these viruses may co-circulate. This study aimed to develop real-time PCR test systems for identifying and differentiating unclassified viruses within the family. In this study, tick suspensions containing phenuiviruses, previously obtained during the screening of ticks from various regions of Russia using pan-phenuivirus primers, were used. Specific primers and probes were designed to differentiate five viruses of genera , , and one unclassified phenuivirus, and their analytical sensitivity and specificity were evaluated. These PCR-based tools provide a robust method for detecting and classifying uncharacterized phenuiviruses, contributing to improved surveillance and understanding their potential epidemiological and epizootological impacts.
该病毒科隶属于某目，包括虫媒病毒和与节肢动物相关的病毒，主要传播媒介为白蛉、蚊子和蜱。历史上，只有白蛉/蚊子传播的本扬病毒与人类疾病有关，但严重发热伴血小板减少综合征（SFTS）的出现凸显了蜱传播的本扬病毒作为人类病原体的潜力。最近发现了一些新的与节肢动物相关的病毒，其中一些尚未分类，这突出了对灵敏检测和鉴别方法的需求，特别是在这些病毒可能共同传播的地区。本研究旨在开发实时PCR检测系统，用于鉴定和区分该病毒科内未分类的病毒。在本研究中，使用了含有本扬病毒的蜱悬液，这些蜱悬液是之前在使用泛本扬病毒引物对俄罗斯不同地区的蜱进行筛查时获得的。设计了特异性引物和探针，以区分属、属、属的五种病毒和一种未分类的本扬病毒，并评估了它们的分析灵敏度和特异性。这些基于PCR的工具为检测和分类未鉴定的本扬病毒提供了一种可靠的方法，有助于加强监测并了解它们潜在的流行病学和动物流行病学影响。
