Instituto de Investigaciones Biológicas del Trópico, Facultad de Medicina Veterinaria y Zootecnia, Universidad de Córdoba, Montería, Colombia.
Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, USA.
Virol J. 2020 May 5;17(1):63. doi: 10.1186/s12985-020-01327-9.
Tick-borne phenuivirus (TBPVs) comprise human and animal viruses that can cause a variety of clinical syndromes ranging from self-limiting febrile illness to fatal haemorrhagic fevers.
Detect Phlebovirus (Family Phenuiviridae) in ticks collected from domestic animals in Córdoba, Colombia.
We collected 2365 ticks from domestic animals in three municipalities of the Department of Cordoba, Colombia in 2016. Ticks were identified and pooled by species for RNA extraction. A nested real-time PCR with specific primers for Phlebovirus and a specific probe for Heartland virus (HRTV) formerly a Phlebovirus, now a Banyangvirus were performed. Also, a conventional nested PCR, with the same specific primers was used to detect other Phleboviruses, with positive reactions indicated by an amplified cDNA fragment of approximately 244 bp determined by gel electrophoresis. These bands were gel-purified and sequenced by the Sanger method.
Using real-time RT-PCR, no positive results for HRTV were found. However, using conventional nested PCR 2.2% (5/229 pools) yielded a product of 244 bp. One positive sample was detected in a pool of Dermacentor nitens ticks collected from a horse, and the four remaining positive pools were from Rhipicephalus microplus collected from cattle. The five positive nucleotide sequences had identities of 93 to 96% compared to a section of the L-segment of Lihan Tick virus, a Phlebovirus originally detected in R. microplus ticks in China. The strongest identity (96-99%) was with Lihan Tick virus detected in R. microplus ticks from Brazil.
This is the first report of viral detection in ticks in Colombia. We detected a Colombian strain of Lihan Tick virus. We recommend expanding the sampling area and carrying out more eco-epidemiological studies related to epidemiological surveillance of viruses on ticks in Colombia.
蜱传病毒（TBPV）包括可引起多种临床综合征的人类和动物病毒，从自限性发热疾病到致命性出血热不等。
在哥伦比亚科尔多瓦省的家畜采集的蜱中检测 Phlebovirus（Phenuiviridae 科）。
我们于 2016 年从哥伦比亚科尔多瓦省的三个城市共采集了 2365 只蜱。对蜱进行了鉴定并按种属进行了汇集，用于 RNA 提取。使用针对 Phlebovirus 的巢式实时 PCR 和针对 Heartland 病毒（HRTV）的特异性探针进行了检测，HRTV 曾是 Phlebovirus，现在是 Banyangvirus。此外，还使用相同的特异性引物进行了常规巢式 PCR 检测，阳性反应通过凝胶电泳确定约 244 bp 的扩增 cDNA 片段指示。这些条带经凝胶纯化并通过 Sanger 法测序。
使用实时 RT-PCR 未发现 HRTV 的阳性结果。然而，使用常规巢式 PCR 检测到 2.2%（5/229 个汇集）产生了 244 bp 的产物。在从一匹马身上采集的 Dermacentor nitens 蜱汇集物中检测到一个阳性样本，其余四个阳性汇集物来自从牛身上采集的 Rhipicephalus microplus。五个阳性核苷酸序列与中国从 R. microplus 蜱中首次检测到的 Lihan Tick virus 的 L 片段的一部分相比具有 93-96%的同一性。与从巴西的 R. microplus 蜱中检测到的 Lihan Tick virus 具有最强的同一性（96-99%）。
这是哥伦比亚首次报告在蜱中检测到病毒。我们检测到了哥伦比亚的 Lihan Tick virus 株。我们建议扩大采样区域，并开展更多与蜱携带病毒的流行病学监测相关的生态流行病学研究。
