海洋真菌KMM 4668菌株内变体的代谢物谱和生物活性差异

Differences in Metabolite Profiles and Bioactivities of Intra-Strain Variants of Marine Fungus KMM 4668.

作者信息

Borkunov Gleb V, Kirichuk Natalya N, Chausova Viktoria E, Popov Roman S, Zhuravleva Olesya I, Chingizova Ekaterina A, Yurchenko Ekaterina A, Isaeva Marina P, Yurchenko Anton N

机构信息

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospect 100-Letiya Vladivostoka, Vladivostok 690022, Russia.

Laboratory of Biologically Active Compounds, Institute of High Technologies and Advanced Materials, Far Eastern Federal University, Vladivostok 690922, Russia.

出版信息

Metabolites. 2025 Jan 27;15(2):77. doi: 10.3390/metabo15020077.

DOI:10.3390/metabo15020077

PMID:39997702

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11857625/

Abstract

During the cultivation of the marine fungus KMM 4668 on solid agar medium, the morphological instability of the strain was observed. As a result of the selection work, five intra-strain variants, named KMM 4711, KMM 4712, KMM 4713, KMM 4714, and KMM 4715, were obtained. The main objectives of this work were to compare the parent strain and its intra-strain variants using multi-locus phylogenetic analysis and to study the UPLC MS metabolite profiles and cytotoxic activities of their extracts. A study of the original strain, KMM 4668, and its intra-strain variants using multi-locus phylogenetic analysis showed that they are sequence identical and belong to . UPLC MS metabolite profiling of fungal extracts revealed 20 compounds, including cladosporin-related polyketides, carotane sesquiterpenoids, andrastine meroterpenoids, and alkaloids. It was shown that the intra-strain variants KMM 4713 and KMM 4714 differed most strongly from the others in the increased production of cladosporin-related compounds, carotanoids, and the alkaloid chrysogin. In addition, the influence of fungal extracts on the viability of four mammalian cell lines was investigated. It has been shown that the intra-strain variants of KMM 4668 may be promising sources of bioactive secondary metabolites.

摘要

在海洋真菌KMM 4668在固体琼脂培养基上培养的过程中，观察到该菌株的形态不稳定性。经过筛选工作，获得了五个菌株内变体，分别命名为KMM 4711、KMM 4712、KMM 4713、KMM 4714和KMM 4715。这项工作的主要目的是使用多位点系统发育分析比较亲本菌株及其菌株内变体，并研究其提取物的超高效液相色谱-质谱代谢物谱和细胞毒性活性。使用多位点系统发育分析对原始菌株KMM 4668及其菌株内变体进行的研究表明，它们的序列相同，属于……真菌提取物的超高效液相色谱-质谱代谢物谱分析揭示了20种化合物，包括枝孢菌素相关的聚酮化合物、胡萝卜烷倍半萜、安拉斯汀杂萜类化合物和生物碱。结果表明，菌株内变体KMM 4713和KMM 4714与其他变体差异最大，它们产生的枝孢菌素相关化合物、类胡萝卜素和生物碱金精的量有所增加。此外，还研究了真菌提取物对四种哺乳动物细胞系活力的影响。结果表明，KMM 4668的菌株内变体可能是生物活性次生代谢物的有前途的来源。