Lu Jing, You Zishen, Zhang Yanhui, Wang Fang, Wang Luanfeng, Xiong Ling, Song Haizhao, Shen Xinchun
College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, China.
J Agric Food Chem. 2025 Mar 12;73(10):5981-5995. doi: 10.1021/acs.jafc.4c11795. Epub 2025 Feb 25.
Dragon fruit offers numerous health benefits and is widely consumed. However, research on its polysaccharides, crucial constituents of the fruit, remains limited. The study aimed to characterize the structural and biological properties of SDFP-2, a polysaccharide isolated from dragon fruit. Structural analyses revealed that SDFP-2, with a molecular mass of 8.08 × 10 Da, consisted of mannose, glucosamine hydrochloride, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose in a molar ratio of 0.181:0.250:16.544:12.762:3.793:0.739:51.014:14.716. SDFP-2 comprises HG-type and RG-I-type pectic structures, along with an arabinogalactan structure characterized by a main chain of →6)-β-D-Galp-(1→6)-β-D-Galp-(1→, with side chains attached at the -3 position of the 1,6-β-galactose residues. Functionally, SDFP-2 exhibited notable hypolipidemic and hypoglycemic properties in HepG2 cell assays, significantly reducing lipid accumulation and enhancing glucose metabolism by restoring key glycolytic enzyme activities. In vitro fermentation with fecal microbiota demonstrated SDFP-2's ability to modulate gut microbial composition, elevating beneficial short-chain fatty acid production, including acetate, propionate, and butyrate. This microbial shift favored SCFA producers, such as and , while diminishing pro-inflammatory bacteria like , underscoring SDFP-2's prebiotic potential. These findings elucidated SDFP-2 as a promising dietary intervention for metabolic regulation and intestinal health enhancement.
火龙果具有诸多健康益处，且被广泛食用。然而，对其果实的关键成分多糖的研究仍然有限。本研究旨在表征从火龙果中分离出的一种多糖SDFP - 2的结构和生物学特性。结构分析表明，SDFP - 2的分子量为8.08×10 Da，由甘露糖、盐酸氨基葡萄糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖组成，摩尔比为0.181:0.250:16.544:12.762:3.793:0.739:51.014:14.716。SDFP - 2包含HG型和RG - I型果胶结构，以及一种阿拉伯半乳聚糖结构，其特征是主链为→6)-β-D-半乳糖-(1→6)-β-D-半乳糖-(1→，侧链连接在1,6-β-半乳糖残基的-3位。在功能上，SDFP - 2在HepG2细胞实验中表现出显著的降血脂和降血糖特性，通过恢复关键糖酵解酶活性显著减少脂质积累并增强葡萄糖代谢。与粪便微生物群的体外发酵表明，SDFP - 2能够调节肠道微生物组成，提高有益短链脂肪酸的产生，包括乙酸、丙酸和丁酸。这种微生物变化有利于短链脂肪酸产生菌，如 和 ，同时减少促炎细菌如 ，突出了SDFP - 2的益生元潜力。这些发现阐明了SDFP - 2作为一种有前景的饮食干预手段，可用于代谢调节和肠道健康改善。
