Ji Xiaolong, Jiang Ke, Liu Yuqing, Zhao Chenyu, Du Jun, Chen Liang, Zhu Zhigang, Li Xiaoqiong
College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China.
Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Foods. 2025 Aug 25;14(17):2954. doi: 10.3390/foods14172954.
Recent studies have increasingly emphasized the regulatory potential of plant-derived polysaccharides on gut microbial composition and metabolic function. Despite this growing interest, investigations focusing specifically on the simulated digestion and fermentation properties of arecanut polysaccharide (PAP1b) remain limited. In this work, we employed the standardized INFOGEST 2.0 protocol to mimic the oral, gastric, and intestinal digestion of PAP1b, followed by 48 h anaerobic fermentation using pooled human fecal samples from healthy adult donors. PAP1b treatment led to a progressive decrease in pH and a substantial elevation in SCFAs levels, notably acetic, propionic, and butyric acids. Simultaneously, PAP1b significantly promoted the growth of SCFA-producing microbial taxa, particularly members of the phylum such as , , , and , while markedly suppressing populations. Metabolomic analysis further indicated that PAP1b intake enhanced bile acid metabolism, suggesting its potential as a prebiotic candidate for improving intestinal health.
最近的研究越来越强调植物源多糖对肠道微生物组成和代谢功能的调节潜力。尽管对此的兴趣日益浓厚，但专门针对槟榔多糖（PAP1b）模拟消化和发酵特性的研究仍然有限。在这项工作中，我们采用标准化的INFOGEST 2.0方案来模拟PAP1b的口腔、胃和肠道消化，然后使用来自健康成年供体的混合人粪便样本进行48小时厌氧发酵。PAP1b处理导致pH值逐渐降低，短链脂肪酸水平大幅升高，尤其是乙酸、丙酸和丁酸。同时，PAP1b显著促进了产生短链脂肪酸的微生物类群的生长，特别是 门的成员，如 、 、 和 ，同时显著抑制 种群。代谢组学分析进一步表明，摄入PAP1b可增强胆汁酸代谢，表明其作为改善肠道健康的益生元候选物的潜力。
