Oshio C, Miyairi M, Watanabe S, Smith C R, Phillips M J
Liver. 1985 Apr;5(2):101-7. doi: 10.1111/j.1600-0676.1985.tb00222.x.
The effect of colchicine on spontaneous in vitro contractions of bile canaliculi was assessed using time-lapse cinephotomicrography. Colchicine 2.5 microM and/or taurocholate 5 microM were added to the culture medium. The experiment was long-term, lasting for 13 h. Frame-by-frame analysis revealed that colchicine, after a lag period of 4 h, suppressed the number of contractions over the remainder of the 13-h experimental period, even in the presence of taurocholate (p less than 0.001). The number of contractions per hour decreased in both the colchicine and combined colchicine-plus-taurocholate-treated groups compared to untreated and taurocholate-treated controls. These results do not necessarily mean that colchicine has an effect on the contractile process per se, since the contractions, when they occurred, appeared normal. It is likely that the canalicular motility events are related to bile secretion. The reduction in contractions after a lag period likely indicates that secretion is impaired, and hence the need for contractions is reduced. These results support the view that for normal canalicular bile secretory function, microtubules as well as a normal microfilament system are required.
采用延时电影显微摄影术评估秋水仙碱对体外胆小管自发收缩的影响。将2.5微摩尔的秋水仙碱和/或5微摩尔的牛磺胆酸盐添加到培养基中。实验为期13小时，是长期实验。逐帧分析显示，秋水仙碱在4小时的延迟期后，在13小时实验期的剩余时间内抑制了收缩次数，即使存在牛磺胆酸盐也是如此（p<0.001）。与未处理和牛磺胆酸盐处理的对照组相比，秋水仙碱处理组和秋水仙碱加牛磺胆酸盐联合处理组每小时的收缩次数均减少。这些结果不一定意味着秋水仙碱本身对收缩过程有影响，因为收缩发生时看起来是正常的。胆小管运动事件可能与胆汁分泌有关。延迟期后收缩次数的减少可能表明分泌受损，因此对收缩的需求减少。这些结果支持这样一种观点，即正常的胆小管胆汁分泌功能需要微管以及正常的微丝系统。
