Phillips M J, Oshio C, Miyairi M, Katz H, Smith C R
Hepatology. 1982 Nov-Dec;2(6):763-8. doi: 10.1002/hep.1840020603.
Living hepatocytes observed under standard methods of cell isolation and culture show frequent active contractions of bile canaliculi when viewed by time-lapse cinephotomicrography. This report gives details of the methods used to show the contractions. In addition, the characteristics of the contraction pattern are defined and analyzed. The contractions are regular, canalicular contraction time (systole) lasts 60 sec, and the most probably interval between contractions is 5 1/2 min. The finding of a regular pattern of contraction suggests an ordered, programmed, and integrated mechanism which entails a high degree of communication and functional coordination between neighboring hepatocytes.
在细胞分离和培养的标准方法下观察到的活肝细胞，通过延时电影显微摄影术观察时，可见胆小管频繁出现主动收缩。本报告详细介绍了用于显示这些收缩的方法。此外，还对收缩模式的特征进行了定义和分析。收缩是有规律的，胆小管收缩时间（收缩期）持续60秒，收缩之间最可能的间隔是5分半钟。发现有规律的收缩模式表明存在一种有序、程序化和整合的机制，这需要相邻肝细胞之间高度的通讯和功能协调。
