Zhang Yunting, Deng Meiyi, Lin Bangyu, Tian Su, Chen Yan, Huang Shan, Lin Yuanxiu, Li Mengyao, He Wen, Wang Yan, Zhang Yong, Chen Qing, Luo Ya, Wang Xiaorong, Gu Xianjie, Tang Haoru
College of Horticulture, Sichuan Agricultural University, Chengdu, 611130, China.
Mianyang Academy of Agricultural Sciences, Mianyang, 621000, China.
BMC Genomics. 2025 Feb 25;26(1):196. doi: 10.1186/s12864-025-11368-5.
As one of the most salt-sensitive crops, strawberry production is severely limited by salt stress. γ--aminobutyric acid (GABA) has been reported to play an important role in the immune response of plants. In this study, the physiological and transcriptomic changes in strawberry seedlings treated with GABA under salt stress were investigated to explore the effect of GABA on salt tolerance. The results showed that exogenous GABA maintained high osmolyte levels, increased antioxidant capacity, and decreased the ROS levels in strawberry leaves under salt stress; the MDA was reduced by 3.27-31.46%, with 10 mM being the most significant effect; the total (Spd + Spm)/ Put ratio was upregulated after GABA treatments. More strikingly, the plants treated with 10 mM GABA significantly increased chlorophyll content and net photosynthetic rate in salt-stressed plants, which was explained by the transcriptomic data showing that the expression levels of most of chlorophyll metabolism and photosynthesis-related genes were upregulated. Furthermore, 38 potential TFs belonging to the WRKY, AP2/ERF, and MYB families were identified that may be positively involved in GABA-induced salt tolerance. Co-expressed network analysis revealed that some of these TFs, such as RAP2.7, WRKY46, and MYB306, were significantly positively correlated with chlorophyll metabolism. These findings provide an important basis for the use of GABA in the breeding of strawberry resistant to salt stress.
作为对盐最敏感的作物之一，草莓生产受到盐胁迫的严重限制。据报道，γ-氨基丁酸（GABA）在植物的免疫反应中起重要作用。本研究调查了盐胁迫下用GABA处理的草莓幼苗的生理和转录组变化，以探讨GABA对耐盐性的影响。结果表明，外源GABA在盐胁迫下维持了较高的渗透溶质水平，提高了抗氧化能力，并降低了草莓叶片中的活性氧水平；丙二醛（MDA）含量降低了3.27%-31.46%，其中10 mM的效果最为显著；GABA处理后，（Spd+Spm）/Put的总比值上调。更引人注目的是，用10 mM GABA处理的植株显著提高了盐胁迫植株的叶绿素含量和净光合速率，转录组数据表明，大多数叶绿素代谢和光合作用相关基因的表达水平上调，对此作出了解释。此外，还鉴定出38个属于WRKY、AP2/ERF和MYB家族的潜在转录因子，它们可能正向参与GABA诱导的耐盐性。共表达网络分析表明，其中一些转录因子，如RAP2.7、WRKY46和MYB306，与叶绿素代谢显著正相关。这些发现为利用GABA培育耐盐草莓品种提供了重要依据。
