Ben-Itzhak J, Levi B Z, Shor R, Lanir A, Bassan H M, Ulitzur S
Mutat Res. 1985 Jun;147(3):107-12. doi: 10.1016/0165-1161(85)90023-8.
The kinetics of the formation of mutagenic metabolites of benzo[a]pyrene (BP) in an isolated perfused rat-liver system have been studied. No genotoxic activity was detected in the perfusate using either the Ames test or the new bioluminescence test for genotoxic agents (BLT). The bile excretion showed strong genotoxic activity especially in the presence of the deconjugation enzymes beta-glucuronidase and arylsulfatase. The BLT was 1000-fold more sensitive than the Ames test in detecting the genotoxic activity in the bile excretion.
已对苯并[a]芘（BP）在离体灌注大鼠肝脏系统中诱变代谢物的形成动力学进行了研究。使用艾姆斯试验或新的遗传毒性剂生物发光试验（BLT）在灌注液中均未检测到遗传毒性活性。胆汁排泄显示出很强的遗传毒性活性，尤其是在存在去结合酶β-葡萄糖醛酸酶和芳基硫酸酯酶的情况下。在检测胆汁排泄中的遗传毒性活性方面，BLT比艾姆斯试验灵敏1000倍。
