利用全基因组亚硫酸氢盐测序（WGBS）鉴定四川白鹅垂体组织中的DNA甲基化差异

Identification of DNA Methylation Differences in Pituitary Tissues of Sichuan White Geese Using Whole-Genome Bisulfite Sequencing (WGBS).

作者信息

Ma Lin, Zhao Xianzhi, A Guoda, Song Tongtong, Wu Meng, Yan Zhihao, Xiao Min, Jiang Wenbo, Gao Yixiao, Wang Haiwei, Chen Zhuping, Zhang Keshan, Xue Jiajia, Luo Yi, Wang Chao, Xie Youhui, Chen Ying, Gao Guangliang, Wang Qigui

机构信息

Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China.

College of Animal Sciences and Technology, Northeast Agricultural University, Harbin 150030, China.

出版信息

Biology (Basel). 2025 Feb 3;14(2):154. doi: 10.3390/biology14020154.

DOI:10.3390/biology14020154

PMID:40001922

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11851436/

Abstract

To explore the impact of epigenetic modifications on egg-laying traits in geese, we employed genome-wide bisulfite sequencing (WGBS) to analyze DNA methylation patterns in pituitary tissues of high-(HYP) and low-yield (LYP) Sichuan White geese. We achieved high-quality sequencing data (mean 19.09 Gb raw reads, 15.49 Gb clean reads, 79.1% unique mapping rate) with a bisulfite conversion efficiency of 99.88%. Comparative analysis revealed 2394 differentially methylated regions (DMRs) and 422 differentially methylated genes (DMGs) between HYP and LYP groups. We identified five key differentially methylated candidate genes (, , , , and ) linked to egg-laying traits in Sichuan White geese. Integrated GO and KEGG enrichment analysis conducted to explore the role of regulatory networks of epigenetic modification on egg-laying traits in Sichuan White geese identified multiple metabolic pathways associated with egg-laying traits (promoting egg transport, ovulation, and yolk protein synthesis and secretion), thus providing a basis for subsequent functional verification.

摘要

为了探究表观遗传修饰对鹅产蛋性状的影响，我们采用全基因组亚硫酸氢盐测序（WGBS）技术分析了高产（HYP）和低产（LYP）四川白鹅垂体组织中的DNA甲基化模式。我们获得了高质量的测序数据（平均原始读长19.09 Gb，clean读长15.49 Gb，唯一映射率79.1%），亚硫酸氢盐转化效率为99.88%。比较分析揭示了HYP组和LYP组之间有2394个差异甲基化区域（DMRs）和422个差异甲基化基因（DMGs）。我们鉴定出了五个与四川白鹅产蛋性状相关的关键差异甲基化候选基因（、、、和）。通过综合GO和KEGG富集分析来探究表观遗传修饰调控网络对四川白鹅产蛋性状的作用，确定了多个与产蛋性状相关的代谢途径（促进卵子运输、排卵以及卵黄蛋白的合成与分泌），从而为后续的功能验证提供了依据。