Luo Xinrong, Liu Zhanwen, Xia Zhanfeng, Luo Xiaoxia, Zhang Juan, Chen Ailiang, Wang Haoxin, Wan Chuanxing, Zhang Lili
State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Science and Technology, Tarim University, Alar 843300, China.
Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Biology (Basel). 2025 Feb 6;14(2):164. doi: 10.3390/biology14020164.
The Taklamakan Desert is an extreme environment supporting a unique and diverse microbial community with significant potential for exploration. Strain TRM70308, isolated from desert soil, shares 98.43% 16S rRNA gene sequence similarity with OF1. Polyphasic taxonomy confirmed TRM70308 as a novel species, named . Genomic analysis revealed that only one of the strain's 25 biosynthetic gene clusters (BGCs) formed a cluster of gene families (CGFs) within the MIBiG database, emphasizing its genomics uniqueness. LC-MS/MS and Feature-Based Molecular Networking (FBMN) identified 33 metabolites across various categories, including alkaloids, saponins, benzoic acids, and benzofurans, most of which remain uncharacterized. Further chemical investigation led to the isolation of one novel compound, aconicarpyrazine C, and four known compounds: thiolutin, dibutyl phthalate, bis(2-ethylhexyl) phthalate, and -acetyltryptamine. Thiolutin exhibited strong activity against five local fungal pathogens that cause plant diseases, with a production yield of 270 mg/L. These results establish a foundation for pilot-scale thiolutin production and its potential development as an antifungal agent for agricultural applications. Our findings highlight deserts as a valuable source of novel actinomycetes and bioactive natural products with immense potential for future research and development.
塔克拉玛干沙漠是一个极端环境，拥有独特多样的微生物群落，具有巨大的探索潜力。从沙漠土壤中分离出的菌株TRM70308与OF1的16S rRNA基因序列相似度为98.43%。多相分类法证实TRM70308为一个新物种，命名为 。基因组分析表明，该菌株的25个生物合成基因簇（BGCs）中只有一个在MIBiG数据库中形成了基因家族簇（CGFs），突出了其基因组的独特性。液相色谱-串联质谱（LC-MS/MS）和基于特征的分子网络（FBMN）鉴定出33种不同类别的代谢产物，包括生物碱、皂苷、苯甲酸和苯并呋喃，其中大多数仍未被表征。进一步的化学研究导致分离出一种新化合物，乌头果嗪C，以及四种已知化合物：硫藤黄素、邻苯二甲酸二丁酯、邻苯二甲酸二（2-乙基己基）酯和N-乙酰色胺。硫藤黄素对五种引起植物病害的本地真菌病原体表现出强大的活性，产量为270 mg/L。这些结果为硫藤黄素的中试生产及其作为农业应用抗真菌剂的潜在开发奠定了基础。我们的研究结果突出了沙漠作为新型放线菌和具有巨大未来研发潜力的生物活性天然产物的宝贵来源。
