CSIRO Agriculture and Food, PO Box 1700, Acton, ACT, Acton 2601, Australia.
CSIRO Microbiomes for One Systems Health Future Science Platform, PO Box 1700, Acton, ACT, Canberra 2601, Australia.
J Agric Food Chem. 2024 Nov 6;72(44):24432-24448. doi: 10.1021/acs.jafc.4c04989. Epub 2024 Oct 23.
species can form beneficial relationships with hosts as endophytes, including the phytopathogen-inhibiting strain, MH191, isolated from wheat plants. Using genomic characterization and untargeted metabolomics, we explored the capacity of strain MH191 to inhibit a range of fungal phytopathogens through the production of secondary metabolites. Complete genome assembly of strain MH191 predicted 24 biosynthetic gene clusters. Secondary metabolite production was assessed following culture on six different media, with the detection of 205 putative compounds. Members of the manumycin family, undecylprodigiosin, and desferrioxamine were identified as the predominant metabolites. Antifungal activity was validated for undecylprodigiosin and manumycin. These compounds were produced from different BGCs, which showed similarity to asukamycin, undecylprodigiosin, and FW0622 gene clusters, respectively. The growth of strain MH191 on different media illustrated the metabolic regulation of these gene clusters and the strain's extended chemical potential, with the asukamycin gene cluster alone, producing a variety of antifungal metabolites. The study highlights the extended chemical capability of strain MH191, which could be exploited as a biological control agent for designing future crop protection solutions.
物种可以与宿主形成有益的关系，包括内生菌，从小麦植物中分离出的抑制植物病原菌的 MH191 菌株。我们使用基因组特征分析和非靶向代谢组学，通过次生代谢产物的产生，探索了 MH191 菌株抑制一系列真菌植物病原菌的能力。MH191 菌株的完整基因组组装预测了 24 个生物合成基因簇。在六种不同的培养基上培养后，评估了次生代谢产物的产生，检测到 205 种假定化合物。曼母菌素家族、十一烷丙基普洛地辛和去铁胺被确定为主要代谢产物。验证了十一烷丙基普洛地辛和曼母菌素的抗真菌活性。这些化合物是由不同的 BGC 产生的，分别与 asukamycin、十一烷丙基普洛地辛和 FW0622 基因簇具有相似性。MH191 菌株在不同培养基上的生长情况说明了这些基因簇的代谢调节以及该菌株的扩展化学潜力，仅 asukamycin 基因簇就产生了多种抗真菌代谢物。该研究突出了 MH191 菌株的扩展化学能力，可将其作为生物防治剂用于设计未来的作物保护解决方案。
