Zou Chengwu, He Fanglian, Li Huinan, Liu Lili, Qiu Zuyang, Dong Weiqing
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, Nanning 530004, China.
Vegetable Research Institute, Guangxi Zhuang Autonomous Region Academy of Agricultural Sciences, Nanning 530007, China.
Biology (Basel). 2025 Feb 8;14(2):173. doi: 10.3390/biology14020173.
The global significance of , a tuber crop rich in nutritional value and starch, prompts further investigation into its corm development.
Previous studies have focused on starch accumulation within the tubers, yet the genetic and proteomic basis of corm expansion remains largely unexplored. This study aims to elucidate the key genes and proteins involved in this process.
We selected 'Lipu Taro No.1' and conducted a longitudinal starch content analysis, full-length transcriptome sequencing, and a proteomic analysis during three distinct stages of corm development.
Our findings reveal a significant increase in both amylose and amylopectin contents as the corm develops, indicating the temporal regulation of starch biosynthesis. The integration of transcriptome and proteomic data identified differentially expressed genes and proteins associated with starch and sucrose metabolism, as well as plant hormone signal transduction.
This study delineates a temporal gene expression pattern that is crucial for starch synthesis and provides insights into the regulatory mechanisms controlling corm expansion and starch deposition, offering valuable references for future molecular breeding strategies to enhance taro yield and quality.
作为一种富含营养价值和淀粉的块茎作物，[作物名称未给出]的全球重要性促使人们进一步研究其球茎发育。
先前的研究集中在块茎内的淀粉积累上，但球茎膨大的遗传和蛋白质组学基础在很大程度上仍未被探索。本研究旨在阐明参与这一过程的关键基因和蛋白质。
我们选择了“荔浦芋1号”，并在球茎发育的三个不同阶段进行了纵向淀粉含量分析、全长转录组测序和蛋白质组分析。
我们的研究结果表明，随着球茎的发育，直链淀粉和支链淀粉含量均显著增加，这表明淀粉生物合成存在时间调控。转录组和蛋白质组数据的整合鉴定出了与淀粉和蔗糖代谢以及植物激素信号转导相关的差异表达基因和蛋白质。
本研究描绘了一种对淀粉合成至关重要的时间基因表达模式，并深入了解了控制球茎膨大及淀粉沉积的调控机制，为未来提高芋头产量和品质的分子育种策略提供了有价值的参考。
