Ogunniran Adefunke Oluwaseun, Dauda Oluwasegun Sodiq, Rotimi Damilare, Jegede Foluso Christopher, Falodun Deborah Joy, Adekunle Precious Oluwaseun
Department of Science Laboratory Technology, Faculty of Science, Ekiti State University, Ado-Ekiti, Ekiti PMB 5363, Nigeria.
Department of Microbiology, Faculty of Science, Federal University, Oye, Nigeria.
Toxicol Rep. 2024 Oct 28;13:101793. doi: 10.1016/j.toxrep.2024.101793. eCollection 2024 Dec.
is a medicinal plant with numerous benefits in different parts of the world. This study evaluated the leaf's nutritional composition, mineral composition, phytochemical constituents, antioxidant properties and antimicrobial properties, and high-performance liquid chromatography (HPLC) using standard methods. The proximate analysis revealed the presence of moisture content (12.14 ± 0.01), ash content (1.05 ± 0.07), crude fat (4.21 ± 0.10), crude protein at (9.78 ± 0.11), crude fiber (2.70 ± 0.32), and carbohydrate content (70.12 ± 0.37). Mineral analysis showed an abundance of potassium (125.18 ± 0.04), followed by magnesium (32.62 ± 0.01), and phosphorus (18.30 ± 0.02). The phytochemical screening revealed the presence of saponins, flavonoids, phenolics, steroids, and alkaloids with 13.25 ± 0.03 mg GAE/g total phenolics and 3.99 ± 0.01 mg QE/g flavonoid contents, respectively. IC values of the scavenging abilities of DPPH, NO radicals, and TBARS were 206.01 g/mL, 347.66 g/mL, and 394.92 g/mL, respectively while the IC value for FRAP was 145.01 g/mL. 14028 and 27853 were most susceptible. At 100 mg/mL, their average zone of inhibition was 18 and 16 mm for ethyl acetate and 15 and 12 mm for methanol, respectively. The minimum inhibitory concentration (MIC) for both isolates was 25 and 50 mg/mL while the leaves were rich in chlorogenic acid, p-coumaric acid, friedelin, quercetin, emodin, cassiarin A, and kaempferol. These results reveal the leaf as a good source of nutrients and also confirm its phenolic antioxidant activity and antimicrobial properties.
是一种在世界不同地区有诸多益处的药用植物。本研究采用标准方法评估了该植物叶子的营养成分、矿物质成分、植物化学成分、抗氧化性能和抗菌性能，以及高效液相色谱法（HPLC）。近似分析显示其水分含量为（12.14±0.01），灰分含量为（1.05±0.07），粗脂肪含量为（4.21±0.10），粗蛋白含量为（9.78±0.11），粗纤维含量为（2.70±0.32），碳水化合物含量为（70.12±0.37）。矿物质分析表明钾含量丰富（125.18±0.04），其次是镁（32.62±0.01）和磷（18.30±0.02）。植物化学筛选显示存在皂苷、黄酮类化合物、酚类、甾体和生物碱，总酚含量为13.25±0.03mg GAE/g，黄酮含量为3.99±0.01mg QE/g。DPPH、NO自由基和TBARS清除能力的IC值分别为206.01μg/mL、347.66μg/mL和394.92μg/mL，而FRAP的IC值为145.01μg/mL。14028和27853最敏感。在100mg/mL时，它们对乙酸乙酯的平均抑菌圈分别为18和16mm，对甲醇的平均抑菌圈分别为15和12mm。两种分离株的最低抑菌浓度（MIC）均为25和50mg/mL，同时叶子富含绿原酸、对香豆酸、木栓酮、槲皮素、大黄素、决明素A和山奈酚。这些结果表明该植物叶子是良好的营养来源，也证实了其酚类抗氧化活性和抗菌性能。
