Wang Xin, Cheng Miaomiao, Liu Jinhu, Guo Yaodong, Zhang Yuxiang, Yuan Yahong, Yue Tianli
College of Health Management, Shangluo University, Shangluo 726000, China.
Shaanxi Union Research Center of University and Enterprise for Healthy and Wellness Industry, Shangluo 726000, China.
Foods. 2025 Feb 7;14(4):549. doi: 10.3390/foods14040549.
Probiotics are considered an effective strategy for relieving DSS-induced colitis. This study investigated the protective effects and mechanisms of , a potential probiotic fungus from Fuzhuan brick tea, on colitis. Supplementation with live 10 spores/mL of H-1 and 10 spores/mL of S-6 significantly improved gut integrity by preventing colon shortening, mucus disruption, and goblet cell depletion. Additionally, it significantly reduced proinflammatory cytokines IL-6 and TNF-α levels, enhanced the expression of tight junction molecules (ZO-1, Claudin-1, E-cadherin, and MUC1) and suppressed the NLRP3 signaling pathway. Live H-1 (10 spores/mL) and S-6 (10 spores/mL) can effectively improve colitis. But the inactivated H-1 did not exhibit effective anti-inflammatory effects and significant interspecies differences. In a word, live low-dose H-1 and high-dose S-6 promise a valuable approach to improving colitis. This research not only enhances our understanding of probiotics and their potential therapeutic uses but also sets the stage for future investigations into the mechanisms of action and clinical utilization of in treating colitis and other gut disorders.
益生菌被认为是缓解葡聚糖硫酸钠（DSS）诱导的结肠炎的有效策略。本研究调查了茯砖茶中一种潜在的益生菌真菌对结肠炎的保护作用及其机制。补充每毫升10个孢子的活态H-1和每毫升10个孢子的活态S-6，通过防止结肠缩短、黏液破坏和杯状细胞减少，显著改善了肠道完整性。此外，它还显著降低了促炎细胞因子白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的水平，增强了紧密连接分子（闭锁小带蛋白1、闭合蛋白1、上皮钙黏蛋白和黏蛋白1）的表达，并抑制了NLRP3信号通路。活态H-1（每毫升10个孢子）和S-6（每毫升10个孢子）可有效改善结肠炎。但灭活的H-1未表现出有效的抗炎作用，且存在显著的种间差异。总之，低剂量活态H-1和高剂量S-6有望成为改善结肠炎的有效方法。本研究不仅增进了我们对益生菌及其潜在治疗用途的理解，也为未来研究其治疗结肠炎和其他肠道疾病的作用机制及临床应用奠定了基础。
