脂联素C1Q和胶原蛋白结构域包含rs266729、细胞周期蛋白依赖性激酶抑制剂2A和2B rs10811661以及信号序列受体亚基1 rs9505118多态性及其与妊娠期糖尿病的关联：罗马尼亚人群的病例对照研究
Adiponectin C1Q and Collagen Domain Containing rs266729, Cyclin-Dependent Kinase Inhibitor 2A and 2B rs10811661, and Signal Sequence Receptor Subunit 1 rs9505118 Polymorphisms and Their Association with Gestational Diabetes Mellitus: A Case-Control Study in a Romanian Population.
作者信息
Muntean Mihai, Mărginean Claudiu, Bernad Elena Silvia, Bănescu Claudia, Nyulas Victoria, Muntean Irina Elena, Săsăran Vladut
机构信息
Department of Obstetrics and Gynecology 2, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, 540142 Târgu Mureș, Romania.
Department of Obstetrics and Gynecology, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania.
出版信息
Int J Mol Sci. 2025 Feb 14;26(4):1654. doi: 10.3390/ijms26041654.
Gestational diabetes mellitus (GDM) and type 2 diabetes mellitus (T2DM) are public health concerns worldwide. These two diseases share the same pathophysiological and genetic similarities. This study aimed to investigate the T2DM known single nucleotide polymorphisms (SNPs) of the adiponectin C1Q and collagen domain containing (), cyclin-dependent kinase inhibitor 2A and 2B (), and signal sequence receptor subunit 1 () genes in a cohort of Romanian GDM pregnant women and perinatal outcomes. DNA was isolated from the peripheral blood of 213 pregnant women with = 71) or without ( = 142) GDM. Afterward, (rs266729), (rs10811661), and (rs9505118) gene polymorphisms were genotyped using TaqMan Real-Time PCR analysis. Women with GDM had a higher pre-pregnancy body mass index (BMI) ( < 0.0001), higher BMI ( < 0.0001), higher insulin resistance homeostatic model assessment (IR-HOMA) ( = 0.0002), higher insulin levels ( = 0.003), and lower adiponectin levels ( = 0.004) at birth compared to pregnant women with normoglycemia. GDM pregnant women had gestational hypertension (GH) more frequently during pregnancy ( < 0.0001), perineal lacerations more frequently during vaginal birth ( = 0.03), and more macrosomic newborns ( < 0.0001) than pregnant women from the control group. We did not find an association under any model (allelic, genotypic, dominant, or recessive) of rs266729, rs10811661, and rs9505118 polymorphisms and GDM. In correlation analysis, we found a weak positive correlation (r = 0.24) between the dominant model GG + CG vs. CC of rs266729 and labor induction failure. In the dominant model TT vs. CC + CT of rs10811661, we found a weak negative correlation between this model and perineal lacerations. Our results suggest that the rs266729, the rs10811661, and the rs9505118 gene polymorphisms are not associated with GDM in a cohort of Romanian pregnant women.
妊娠期糖尿病（GDM）和2型糖尿病（T2DM）是全球关注的公共卫生问题。这两种疾病在病理生理和遗传方面具有相同的相似性。本研究旨在调查罗马尼亚妊娠期糖尿病孕妇队列中脂联素C1Q和胶原结构域包含基因（）、细胞周期蛋白依赖性激酶抑制剂2A和2B基因（）以及信号序列受体亚基1基因（）的2型糖尿病已知单核苷酸多态性（SNP）与围产期结局。从213名患有（ = 71）或未患有（ = 142）GDM的孕妇外周血中分离DNA。随后，使用TaqMan实时荧光定量PCR分析对基因多态性（rs266729）、基因多态性（rs10811661）和基因多态性（rs9505118）进行基因分型。与血糖正常的孕妇相比，患有GDM的孕妇孕前体重指数（BMI）更高（ < 0.0001），孕期BMI更高（ < 0.0001），胰岛素抵抗稳态模型评估（IR-HOMA）更高（ = 0.0002），出生时胰岛素水平更高（ = 0.003），脂联素水平更低（ = 0.004）。与对照组孕妇相比，患有GDM的孕妇孕期更频繁发生妊娠期高血压（GH）（ < 0.0001），阴道分娩时更频繁发生会阴裂伤（ = 0.03），且巨大儿更多（ < 0.0001）。我们未发现基因多态性rs266729、基因多态性rs10811661和基因多态性rs9505118的任何模型（等位基因、基因型、显性或隐性）与GDM之间存在关联。在相关性分析中，我们发现rs266729的显性模型GG + CG与CC之间与引产失败存在弱正相关（r = 0.24）。在rs10811661的显性模型TT与CC + CT之间，我们发现该模型与会阴裂伤之间存在弱负相关。我们的结果表明，在罗马尼亚孕妇队列中，基因多态性rs266729、基因多态性rs10811661和基因多态性rs9505118与GDM无关。
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