Pawlik Andrzej, Teler Joanna, Maciejewska Agnieszka, Sawczuk Marek, Safranow Krzysztof, Dziedziejko Violetta
Department of Physiology, Pomeranian Medical University, Powstancow Wlkp. 72, 70-111, Szczecin, Poland.
Department of Obstetrics and Gynecology, County Hospital, Zielona Gora, Poland.
J Assist Reprod Genet. 2017 Apr;34(4):511-516. doi: 10.1007/s10815-016-0866-2. Epub 2017 Jan 3.
Gestational diabetes mellitus (GDM) is the glucose intolerance occurring during pregnancy. The prevalence of GDM is increased in obese women. Leptin and adiponectin are adipokines that play an important role in the regulation of insulin secretion and glucose and lipid metabolism. The aim of this study was to examine the association between adiponectin and leptin gene polymorphisms and the development of GDM.
This case-control study included 204 pregnant women with GDM and 207 pregnant women with normal glucose tolerance (NGT). The diagnosis of GDM was based on a 75-g oral glucose tolerance test (OGTT) at 24-28 weeks' gestation. To discriminate the ADIPOQ rs266729, rs1501299 and LEP rs2167270 alleles, TaqMan® Pre-Designed SNP Genotyping Assays were used.
There was a statistically significant association between the ADIPOQ rs266729 gene polymorphism and GDM. Among women with GDM, a higher prevalence of the G allele was observed (GG and CG genotypes). Multivariate logistic regression analysis, taking into account age, BMI before pregnancy, past pregnancies and the ADIPOQ rs266729 gene polymorphism, revealed that the presence of a G allele is an independent risk factor for GDM. Moreover, there was the association between the LEP rs2167270 polymorphism and the requirement for daily insulin, which was significantly higher in women with the A allele (AA and GA genotypes).
The results of our study suggest an association between adiponectin gene rs266729 as well as leptin gene rs2167270 polymorphisms and GDM.
妊娠期糖尿病（GDM）是孕期出现的葡萄糖不耐受情况。肥胖女性中GDM的患病率有所增加。瘦素和脂联素是脂肪因子，在胰岛素分泌及糖脂代谢调节中发挥重要作用。本研究旨在探讨脂联素和瘦素基因多态性与GDM发生之间的关联。
本病例对照研究纳入了204例患有GDM的孕妇和207例糖耐量正常（NGT）的孕妇。GDM的诊断基于妊娠24 - 28周时的75克口服葡萄糖耐量试验（OGTT）。为鉴别ADIPOQ基因rs266729、rs1501299以及LEP基因rs2167270的等位基因，使用了TaqMan®预设计SNP基因分型检测方法。
ADIPOQ基因rs266729多态性与GDM之间存在统计学显著关联。在患有GDM的女性中，观察到G等位基因（GG和CG基因型）的患病率较高。多因素逻辑回归分析，综合考虑年龄、孕前BMI、既往妊娠情况以及ADIPOQ基因rs266729多态性，显示G等位基因的存在是GDM的独立危险因素。此外，LEP基因rs2167270多态性与每日胰岛素需求之间存在关联，A等位基因（AA和GA基因型）的女性中这一需求显著更高。
我们的研究结果表明脂联素基因rs266729以及瘦素基因rs2167270多态性与GDM之间存在关联。
