Marconi P, Bistoni F, Tissi L, Mosci L, Cenci E, Pitzurra M, Lusian F
Boll Ist Sieroter Milan. 1979 May 31;58(2):133-40.
The Authors carried out a research work on problems relating to technical aspects to the tetanus immunity tests. Some interesting conclusions have been reached: - Purified and diluted tetanus toxin is best preserved at - 20 degrees C, in pH 7.4 physiological salt solution added with 1% peptone. - Vaccination in small laboratory animals must be done injecting adsorbed anatoxin by a microsyringe, in order to avoid dilution of the original compound. - Protection test using low, i.v. toxin doses is more reliable than serological tests in assessing the acquired resistance level in immuno-prophylaxed animals. - Passive haemagglutination test is much more sensitive when done with turkey instead of sheep red blood cells.
作者们针对破伤风免疫测试的技术方面相关问题开展了一项研究工作。得出了一些有趣的结论：- 纯化并稀释后的破伤风毒素最好保存在-20摄氏度，置于添加了1%蛋白胨的pH 7.4生理盐溶液中。- 在小型实验动物中进行疫苗接种时，必须使用微量注射器注射吸附类毒素，以避免原始化合物被稀释。- 在评估免疫预防动物的获得性抵抗力水平时，使用低剂量静脉注射毒素的保护试验比血清学试验更可靠。- 用火鸡红细胞而非绵羊红细胞进行被动血凝试验时，其灵敏度要高得多。
