人血清和浓缩尿中促红细胞生成素的生物测定与放射免疫测定之间的相关性

Correlation between bioassay and radioimmunoassay for erythropoietin in human serum and urine concentrates.

作者信息

Cohen R A, Clemons G, Ebbe S

出版信息

Proc Soc Exp Biol Med. 1985 Jul;179(3):296-9. doi: 10.3181/00379727-179-42099.

DOI:10.3181/00379727-179-42099

Abstract

Both immunoreactive erythropoietin (Ep) and biologically active Ep were measured in 23 samples of human serum and 21 concentrates of human urine. Immunoreactive Ep was measured by radioimmunoassay (RIA). Biological activity was determined in the plethoric mouse bioassay in which 59Fe incorporation was converted to units of Ep from standard reference curves. Low values for Ep were determined from standard curves plotted as probits to improve sensitivity for levels of Ep as low as 30 mU/ml. Ep levels in 35 samples ranged between 30 and 1000 mU/ml by both assays; in 9 samples Ep was 15.2-37.5 mU/ml by RIA but was not detectable by bioassay. Analysis of the data for the 35 samples in which Ep could be measured by both assays showed a strong correlation between the values obtained by the two assays. These results indicate that the RIA used in these experiments detects biologically active Ep in human serum and urine when it is present in amounts only moderately higher than normal. The ultrafiltration method used for preparation of urine samples was effective in concentrating Ep in some urines, but the results were too erratic and nonquantitative to permit its use as a method for quantifying human urinary Ep excretion.

摘要

在23份人血清样本和21份人尿浓缩物中测定了免疫反应性促红细胞生成素（Ep）和生物活性Ep。免疫反应性Ep通过放射免疫测定法（RIA）进行测定。生物活性通过多血质小鼠生物测定法确定，其中从标准参考曲线将59Fe掺入转化为Ep单位。通过绘制概率单位的标准曲线确定低Ep值，以提高对低至30 mU/ml的Ep水平的灵敏度。两种测定方法显示，35份样本中的Ep水平在30至1000 mU/ml之间；9份样本通过RIA测定的Ep为15.2 - 37.5 mU/ml，但生物测定法未检测到。对35份可通过两种测定方法测量Ep的样本的数据进行分析，结果显示两种测定方法所得值之间存在强相关性。这些结果表明，这些实验中使用的RIA在人血清和尿液中生物活性Ep含量仅略高于正常水平时能够检测到它。用于制备尿液样本的超滤方法在某些尿液中有效浓缩了Ep，但结果过于不稳定且无法定量，因此不能用作定量人尿Ep排泄的方法。