Li Yue, Tang Fengru, Luo Yumin
Department of Education, Xuanwu Hospital, Capital Medical University, Beijing, China.
Department of Anatomy, National University of Singapore, Singapore.
Brain Circ. 2024 Dec 28;10(4):343-353. doi: 10.4103/bc.bc_27_24. eCollection 2024 Oct-Dec.
When a seizure occurs, the distribution of purine receptors in different cell types at various time points remains poorly understood. Our literature review revealed that P2X7, P2Y6, and P2Y12 are expressed in different cells during epilepsy pathogenesis. Therefore, we studied the protein expression patterns of the purinergic receptors P2X7, P2Y6, and P2Y12 in the normal mice hippocampus, as well as during or after pilocarpine-induced status epilepticus (DPISE or APISE).
Immunohistochemical staining and double-labeling immunofluorescence staining were used to study the cellular distribution of various purinergic receptors across several groups: control, 2-hour DPISE, 1-day APISE, 2-day APISE, 3-day APISE, and 1-week APISE.
In the normal mouse brain, P2X7, P2Y6, and P2Y12 were predominantly expressed in the neurons. Microglia and astrocytes were found to express these receptors at the onset of seizures. Immunofluorescence analysis showed that P2X7 and P2Y12 are expressed in microglia, whereas P2Y6 is mainly expressed in astrocytes.
Different purinergic receptors are expressed in neurons, microglia, and astrocytes, mediate their interactions, and are involved in epileptogenesis.
癫痫发作时，嘌呤受体在不同细胞类型中不同时间点的分布仍不清楚。我们的文献综述显示，P2X7、P2Y6和P2Y12在癫痫发病机制中的不同细胞中表达。因此，我们研究了嘌呤能受体P2X7、P2Y6和P2Y12在正常小鼠海马以及匹鲁卡品诱导的癫痫持续状态期间及之后（DPISE或APISE）的蛋白表达模式。
采用免疫组织化学染色和双标免疫荧光染色研究不同组（对照组、2小时DPISE、1天APISE、2天APISE、3天APISE和1周APISE）中各种嘌呤能受体的细胞分布。
在正常小鼠脑中，P2X7、P2Y6和P2Y12主要在神经元中表达。在癫痫发作开始时，发现小胶质细胞和星形胶质细胞表达这些受体。免疫荧光分析显示，P2X7和P2Y12在小胶质细胞中表达，而P2Y6主要在星形胶质细胞中表达。
不同的嘌呤能受体在神经元、小胶质细胞和星形胶质细胞中表达，介导它们之间的相互作用，并参与癫痫发生。
