Tong Xinglai, Jiang Tuohao, Yang Jiaying, Song Ying, Ao Qi, Tang Jun, Zhang Ling
Department of Polymer Science, College of Chemistry, Jilin University, Changchun, 130012, China.
Key Laboratory of Pathobiology, Ministry of Education, And Department of Biomedical Science, College of Basic Medical Sciences, Jilin University, Changchun, 130021, China.
Biosens Bioelectron. 2025 Jun 1;277:117307. doi: 10.1016/j.bios.2025.117307. Epub 2025 Feb 25.
Continuous Glucose Monitoring (CGM) device was a kind of based on flexible electrode interstitial fluid (ISF) implantation that used electrochemical methods to track blood glucose fluctuations, which made continuous real-time glucose monitoring and personalized blood glucose management increasingly possible. However, when the electrode of CGM in the body fluid environment for a long time, the occurrence of bio-fouling will lead to CGM signal deviation, service life reduction, accuracy decline and other problems. Therefore, in this paper, we constructed a new strategy that provided a well-defined, anti-biofilm coating based and integrated smartphone-controlled wearable microneedle system CGM (acCGM) that can significantly improve accuracy during use and potentially extend service life. In vivo ISF blood glucose monitoring experiment, compared with the commercial blood glucose meter, the acCGM system can accurately monitor the blood glucose level of healthy rats for 21 days. Comparing the two kinds of CGM, it can be found that the MARD of coated CGM within 21 days was 9.69%, and that of uncoated CGM was 16.75%, indicating that the coating had a more obvious anti-biofouling effect. Notably, at 14-21 days after implantation, the MARD of the CGM with the anti-biofouling coating remained at 11.67%, indicating that the acCGM also had the potential to work longer. In addition, the acCGM system with anti-biofouling coating also offered low cost, biosafety, high accuracy and no need for manual calibration.
连续血糖监测（CGM）设备是一种基于柔性电极的组织间液（ISF）植入式设备，它采用电化学方法追踪血糖波动，这使得连续实时血糖监测和个性化血糖管理越来越成为可能。然而，当CGM的电极长时间处于体液环境中时，生物污垢的出现会导致CGM信号偏差、使用寿命缩短、准确性下降等问题。因此，在本文中，我们构建了一种新策略，提供了一种基于明确的抗生物膜涂层且集成了智能手机控制的可穿戴微针系统CGM（acCGM），其在使用过程中可显著提高准确性，并有可能延长使用寿命。在体内ISF血糖监测实验中，与商用血糖仪相比，acCGM系统能够准确监测健康大鼠的血糖水平达21天。比较这两种CGM可以发现，有涂层的CGM在21天内的平均绝对相对偏差（MARD）为9.69%，无涂层的CGM为16.75%，这表明该涂层具有更明显的抗生物污垢效果。值得注意的是，在植入后14 - 21天，具有抗生物污垢涂层的CGM的MARD保持在11.67%，这表明acCGM也有工作更长时间的潜力。此外，具有抗生物污垢涂层的acCGM系统还具有低成本、生物安全性高、准确性高且无需手动校准的特点。
