Sun Jing, Dai Wanqing, Guo Qiang, Gao Yaru, Chen Jiayu, Chen Jian Lin, Mao Guozhu, Sun Hongyan, Peng Yung-Kang
Department of Applied Science, School of Science and Technology, Hong Kong Metropolitan University, Homantin, Kowloon, Hong Kong Special Administrative Region of China.
Department of Applied Science, School of Science and Technology, Hong Kong Metropolitan University, Homantin, Kowloon, Hong Kong Special Administrative Region of China; State Key Laboratory of Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region of China; Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057, China.
Biosens Bioelectron. 2025 Jun 1;277:117303. doi: 10.1016/j.bios.2025.117303. Epub 2025 Feb 23.
Sweat, a vital metabolic product in the human body, contains valuable biomarkers that reflect human conditions. Among these, lactate concentration serves as a significant indicator of human physiological states. In this study, we present an innovative self-powered wearable electrochemical sensor designed for real-time lactate detection in human sweat. This sensor utilizes a composite conductive hydrogel medium, showcasing its potential in monitoring and assessing human health. The sensor incorporates two key components: the lactate oxidase/reduced graphene oxide/carbon cloth electrode (LOx/rGO/CCE) as the anode and the bilirubin oxidase/reduced graphene oxide/carbon cloth electrode (BOx/rGO/CCE) as the cathode. These electrodes are integrated into a substrate comprising a conductive hydrogel composed of Poly (3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) and hydrophilic polyurethane (HPU). The sensor's performance was evaluated. The linear detection range spans from 10 nM to 50 mM, with an impressive detection limit of 4.38 nM, demonstrating its high sensitivity and selectivity towards lactate detection with long-term stability. Additionally, this sensor has been successfully applied to real-time monitor lactate concentration on athletes' skin by combining it with self-made equipment and smartphones. The test results demonstrate minimal error compared to the results obtained from high-performance liquid chromatography. This technology opens up a valuable tool for monitoring and assessing human physiological conditions and new possibilities for advancements in health management, sports monitoring, and medical diagnostics.
汗液是人体重要的代谢产物，含有反映人体状况的有价值生物标志物。其中，乳酸浓度是人体生理状态的重要指标。在本研究中，我们展示了一种创新的自供电可穿戴式电化学传感器，用于实时检测人体汗液中的乳酸。该传感器采用复合导电水凝胶介质，展现出其在监测和评估人体健康方面的潜力。该传感器包含两个关键组件：作为阳极的乳酸氧化酶/还原氧化石墨烯/碳布电极（LOx/rGO/CCE）和作为阴极的胆红素氧化酶/还原氧化石墨烯/碳布电极（BOx/rGO/CCE）。这些电极被集成到一个由聚（3,4 - 乙撑二氧噻吩）：聚苯乙烯磺酸盐（PEDOT:PSS）和亲水性聚氨酯（HPU）组成的导电水凝胶构成的基底中。对该传感器的性能进行了评估。其线性检测范围为10 nM至50 mM，检测限低至4.38 nM，显示出其对乳酸检测具有高灵敏度、高选择性以及长期稳定性。此外，通过将该传感器与自制设备和智能手机相结合，已成功应用于实时监测运动员皮肤表面的乳酸浓度。测试结果表明，与高效液相色谱法所得结果相比，误差极小。这项技术为监测和评估人体生理状况开辟了一种有价值的工具，并为健康管理、运动监测和医学诊断的进步带来了新的可能性。
