Lu Haozi, Wang Junhua, Wu Ziyi, Yang Meiqing, Zhou Wei, Li Ye, Li Huimin, Zhang Yang, Yang Jifei, Yu Gang, Liu Song
Institute of Chemical Biology and Nanomedicine (ICBN), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
Department of Orthopaedics, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.
Talanta. 2025 Apr 1;285:127423. doi: 10.1016/j.talanta.2024.127423. Epub 2024 Dec 22.
Sweat lactate levels are closely related to an individual's physiological state and serve as critical indicators for assessing exercise intensity, muscle fatigue, and certain pathological conditions. Screen-printed electrodes (SPEs) offer a promising avenue for the development of low-cost, high-performance wearable devices for electrochemical sweat analysis. The material composition of SPEs significantly impacts their detection sensitivity and stability. In this study, we designed a screen-printed carbon electrode (SPCE) modified with TiCT Polydopamine (PDA), and silver nanoparticles (AgNPs) (TiCT-PDA-AgNPs) for lactate detection in sweat. The accordion-like structure of TiCT provides a large specific surface area and exceptional electrical conductivity. PDA, acting as both a reducing agent and binder, supports the in-situ formation of AgNPs on the TiCT nanosheets. These AgNPs prevent the restacking of TiCT layers, further improving conductivity. The sensor exhibited sensitivities of 0.145 μA mM, with limit of detection (LOD) of 0.181 mM (S/N = 3) in phosphate-buffered saline (PBS), meeting the requirements for for sweat lactate detection. The sensor was integrated into a wearable micro-electrochemical platform paired with a custom Android application for real-time sweat analysis. Testing on human sweat demonstrated the platform's potential for practical fitness monitoring and healthcare diagnostics applications.
汗液乳酸水平与个体的生理状态密切相关，是评估运动强度、肌肉疲劳和某些病理状况的关键指标。丝网印刷电极（SPEs）为开发用于电化学汗液分析的低成本、高性能可穿戴设备提供了一条有前景的途径。SPEs的材料组成对其检测灵敏度和稳定性有显著影响。在本研究中，我们设计了一种用TiCT、聚多巴胺（PDA）和银纳米颗粒（AgNPs）修饰的丝网印刷碳电极（SPCE）（TiCT-PDA-AgNPs）用于汗液中乳酸的检测。TiCT的手风琴状结构提供了大的比表面积和优异的导电性。PDA作为还原剂和粘合剂，支持在TiCT纳米片上原位形成AgNPs。这些AgNPs防止TiCT层的重新堆叠，进一步提高导电性。该传感器在磷酸盐缓冲盐水（PBS）中的灵敏度为0.145 μA mM，检测限（LOD）为0.181 mM（S/N = 3），满足汗液乳酸检测的要求。该传感器被集成到一个可穿戴微电化学平台中，并与一个定制的安卓应用程序配对用于实时汗液分析。对人体汗液的测试证明了该平台在实际健身监测和医疗诊断应用中的潜力。
