Matsuzaki Ryoma, Matsuoka Teruki, Nakanishi Kazuki, Tani Akira, Kakimoto Shogo, Kato Yuki, Kawatani Takuya, Nakagawa Sae, Baba Yoshitake, Kobayashi Makoto, Takihara Takanobu, Sakakima Harutoshi
Department of Physical Therapy, School of Health Sciences, Faculty of Medicine, Kagoshima University, Kagoshima, Japan.
Central Research Institute, ITO EN, Ltd., 21 Mekami, Makinohara, Shizuoka 421-0516, Japan.
Exp Gerontol. 2025 Apr;202:112720. doi: 10.1016/j.exger.2025.112720. Epub 2025 Feb 25.
Sarcopenia negatively affects the quality of life and health of older individuals. Physical exercise is a standard treatment for sarcopenia. Recently, the potential benefits of green tea catechins (GTCs) in sarcopenia have gained considerable attention. In this study, we investigated the effects of a combination of GTCs and physical exercise on the symptoms and pathologies of sarcopenia using male senescence-accelerated mouse prone 8 (SAMP8). These mice were divided into four groups: control, GTCs, exercise (Ex), and GTCs + Ex. GTC-fed mice were fed a diet containing 0.33 % GTCs. The mice were subjected to exercise training (voluntary wheel running) for 12 weeks, from 5 to 8 months of age, and grip strength and gastrocnemius muscle alterations were investigated. SAMP8 mice exhibited symptoms and pathologies of sarcopenia, including loss of muscle mass, decreased grip strength, reduced mitochondrial capacity, increased oxidative stress, reduced number of satellite cells, and an increased ratio of 5-bromo-2'-deoxyuridine (BrdU)-positive nuclei located within the muscle cells in the aged muscle. The GTCs and/or Ex groups showed improved symptoms and pathologies of sarcopenia. In addition, the GTCs + Ex group exhibited enhanced mitochondrial capacity, myogenic differentiation, and maturation in aged skeletal muscle than that observed in the GTCs group. Our findings suggested that GTCs and/or Ex are effective in ameliorating several age-related changes in muscle morphology and function. Notably, GTCs intake, together with habitual exercise, may enhance the beneficial effects on the symptoms and pathologies of sarcopenia in aged muscle.
肌肉减少症对老年人的生活质量和健康产生负面影响。体育锻炼是治疗肌肉减少症的标准方法。最近，绿茶儿茶素（GTCs）在肌肉减少症中的潜在益处受到了广泛关注。在本研究中，我们使用雄性衰老加速易患8型小鼠（SAMP8），研究了GTCs与体育锻炼相结合对肌肉减少症症状和病理变化的影响。这些小鼠被分为四组：对照组、GTCs组、运动组（Ex）和GTCs + Ex组。给予GTCs的小鼠喂食含有0.33% GTCs的饮食。小鼠在5至8个月大时进行了12周的运动训练（自愿轮转跑步），并研究了握力和腓肠肌的变化。SAMP8小鼠表现出肌肉减少症的症状和病理变化，包括肌肉质量损失、握力下降、线粒体功能降低、氧化应激增加、卫星细胞数量减少以及老年肌肉中位于肌肉细胞内的5-溴-2'-脱氧尿苷（BrdU）阳性细胞核比例增加。GTCs组和/或Ex组的肌肉减少症症状和病理变化有所改善。此外，与GTCs组相比，GTCs + Ex组在老年骨骼肌中表现出线粒体功能增强、成肌分化和成熟。我们的研究结果表明，GTCs和/或Ex可有效改善与年龄相关的肌肉形态和功能变化。值得注意的是，摄入GTCs并结合习惯性运动，可能会增强对老年肌肉中肌肉减少症症状和病理变化的有益影响。
