Dong Xu, Xiang Yanghui, Shen Ping, Xiao Yonghong, Zhang Ying
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Int J Antimicrob Agents. 2025 May;65(5):107484. doi: 10.1016/j.ijantimicag.2025.107484. Epub 2025 Feb 27.
Members of the genus Providencia are typically opportunistic pathogens that can cause severe infections in immunocompromised hosts. This study identified two Providencia strains, SKLX146130 and SKLX074055, representing novel species designated as Providencia zhejiangensis sp. nov. and Providencia xihuensis sp. nov., respectively. Both strains were isolated from clinical samples, with P. zhejiangensis displaying extensive drug resistance. Our analysis revealed that P. xihuensis is predominantly from China, whereas P. zhejiangensis has a global presence. Meanwhile, our analyses resolved Providencia huashanensis as a later heterotypic synonym of Providencia xianensis. Notably, the strain SKLX146130 from P. zhejiangensis, characterized by carrying multiple acquired resistance determinants including the carbapenemase gene bla, belongs to a zoonotic subclade. The genomic analyses of the P. zhejiangensis strain SKLX146130 and Providencia xihuensis strain SKLX074055 showed 79.76-84.55% and 79.85-95.85% average nucleotide identity (ANI), respectively, with other Providencia type strains. In silico DNA-DNA hybridization (dDDH) values ranged from 20.7 to 26.2% and 21.3-64.8%, respectively. These results, along with phenotypic characterization and phylogenetic evidence, confirm that SKLX146130 and SKLX074055 represent two distinct novel species within the genus Providencia. Consequently, we propose the names Providencia zhejiangensis sp. nov. and Providencia xihuensis sp. nov. for these species and call for monitoring these species in clinical infections.
普罗威登斯菌属的成员通常是机会致病菌，可在免疫功能低下的宿主中引起严重感染。本研究鉴定出两株普罗威登斯菌，SKLX146130和SKLX074055，分别代表新命名的浙江普罗威登斯菌（Providencia zhejiangensis sp. nov.）和西湖普罗威登斯菌（Providencia xihuensis sp. nov.）两个新物种。这两株菌均从临床样本中分离得到，浙江普罗威登斯菌表现出广泛的耐药性。我们的分析表明，西湖普罗威登斯菌主要来自中国，而浙江普罗威登斯菌在全球均有分布。同时，我们的分析确定华山普罗威登斯菌（Providencia huashanensis）是西安普罗威登斯菌（Providencia xianensis）的晚出异名。值得注意的是，来自浙江普罗威登斯菌的菌株SKLX146130携带包括碳青霉烯酶基因bla在内的多个获得性耐药决定簇，属于人畜共患亚分支。浙江普罗威登斯菌菌株SKLX146130和西湖普罗威登斯菌菌株SKLX074055的基因组分析显示，与其他普罗威登斯菌模式菌株的平均核苷酸同一性（ANI）分别为79.76 - 84.55%和79.85 - 95.85%。电子DNA - DNA杂交（dDDH）值分别为20.7%至26.2%和21.3 - 64.8%。这些结果，连同表型特征和系统发育证据，证实SKLX146130和SKLX074055代表普罗威登斯菌属内两个不同的新物种。因此，我们为这些物种提议命名为浙江普罗威登斯菌（Providencia zhejiangensis sp. nov.）和西湖普罗威登斯菌（Providencia xihuensis sp. nov.），并呼吁在临床感染中监测这些物种。
