Juneja Punita, Lazzaro Brian P
Department of Entomology, Cornell University, Ithaca, NY 14853, USA.
Int J Syst Evol Microbiol. 2009 May;59(Pt 5):1108-11. doi: 10.1099/ijs.0.000117-0.
Multiple isolates of the genus Providencia were obtained from the haemolymph of wild-caught Drosophila melanogaster fruit flies. Sixteen isolates were distinguished from the six previously described species based on 16S rRNA gene sequences. These isolates belonged to two distinct groups, which we propose each comprise previously undescribed species. Two isolates, designated A(T) and B(T), were characterized by DNA sequences of the fusA, lepA, leuS, gyrB and ileS housekeeping genes, whole-genome DNA-DNA hybridizations with their nearest relatives and utilization of substrates for metabolism. The closest phylogenetic relatives of strain A(T) are strain B(T) (86.9 % identity for the housekeeping genes) and Providencia stuartii DSM 4539(T) (86.0 % identity). The closest phylogenetic relatives of strain B(T) are strain A(T) (86.9 % identity) and P. stuartii DSM 4539(T) (86.6 % identity). The type strains of described species in this genus shared between 84.1 and 90.1 % identity for these sequences. DNA-DNA hybridization between the strain pairs A(T)-B(T), A(T)-P. stuartii DSM 4539(T) and B(T)-P. stuartii DSM 4539(T) all resulted in less than 25 % relatedness. In addition, patterns of utilization of amygdalin, arbutin, aesculin, salicin, d-sorbitol, trehalose, inositol, d-adonitol and d-galactose distinguish strains A(T) and B(T) from other members of this genus. Strains A(T) and B(T) therefore represent novel species, for which the names Providencia sneebia sp. nov. (type strain A(T) =DSM 19967(T) =ATCC BAA-1589(T)) and Providencia burhodogranariea sp. nov. (type strain B(T) =DSM 19968(T) =ATCC BAA-1590(T)) are proposed.
从野生捕获的黑腹果蝇的血淋巴中获得了多个普罗威登斯菌属的分离株。基于16S rRNA基因序列，从先前描述的6个物种中区分出了16个分离株。这些分离株属于两个不同的组，我们认为每组都包含以前未描述过的物种。两个分离株，分别命名为A(T)和B(T)，通过fusA、lepA、leuS、gyrB和ileS管家基因的DNA序列、与它们最近亲缘种的全基因组DNA-DNA杂交以及代谢底物的利用情况进行了特征描述。菌株A(T)最接近的系统发育亲缘种是菌株B(T)（管家基因的同一性为86.9%）和斯氏普罗威登斯菌DSM 4539(T)（同一性为86.0%）。菌株B(T)最接近的系统发育亲缘种是菌株A(T)（同一性为86.9%）和斯氏普罗威登斯菌DSM 4539(T)（同一性为86.6%）。该属中已描述物种的模式菌株在这些序列上的同一性在84.1%至90.1%之间。菌株对A(T)-B(T)、A(T)-斯氏普罗威登斯菌DSM 4539(T)和B(T)-斯氏普罗威登斯菌DSM 4539(T)之间的DNA-DNA杂交结果显示相关性均小于25%。此外，扁桃苷、熊果苷、七叶苷、水杨苷、d-山梨醇、海藻糖、肌醇、d-阿东醇和d-半乳糖的利用模式将菌株A(T)和B(T)与该属的其他成员区分开来。因此，菌株A(T)和B(T)代表了新的物种，为此提出了新名称斯氏普罗威登斯菌新种（模式菌株A(T)=DSM 19967(T)=ATCC BAA-1589(T)）和嗜褐粒普罗威登斯菌新种（模式菌株B(T)=DSM 19968(T)=ATCC BAA-1590(T)）。
