Hao Jing-Wen, Wang Wei-Ting, Chen Nai-Dong, Shen Yan
College of Biotechnology and Pharmaceutical Engineering, West Anhui University, 237012 Lu'an, Anhui, China; Anhui Province Key Laboratory for Quality Evaluation and Improvement of Traditional Chinese Medicine, 237012 Lu'an, Anhui, China; Anhui Engineering Technology Center for Conservation and Utilization of Traditional Chinese Medicine Resource, 237012 Lu'an, Anhui, China; Lu'an City Laboratory for Quality Evaluation and Improvement of Traditional Chinese Medicine, 237012 Lu'an, Anhui, China; School of Life Science, Jiangsu Normal University, 221116 Xuzhou, Jiangsu, China.
College of Biotechnology and Pharmaceutical Engineering, West Anhui University, 237012 Lu'an, Anhui, China; Anhui Province Key Laboratory for Quality Evaluation and Improvement of Traditional Chinese Medicine, 237012 Lu'an, Anhui, China; Anhui Engineering Technology Center for Conservation and Utilization of Traditional Chinese Medicine Resource, 237012 Lu'an, Anhui, China; College of Pharmacy, Anhui University of Chinese Medicine, 230012 Hefei, Anhui, China; Lu'an City Laboratory for Quality Evaluation and Improvement of Traditional Chinese Medicine, 237012 Lu'an, Anhui, China.
Food Chem. 2025 Jun 15;477:143568. doi: 10.1016/j.foodchem.2025.143568. Epub 2025 Feb 27.
The novel application of a combined screening method, utilizing AAPH, ABTS, and FRAP assays with HPLC, is first used for screening natural antioxidants in Green Calyx plums (GCP). This method was assumed that the peak areas of compounds with antioxidant activity in HPLC chromatograms would be significantly reduced or disappeared after incubating with the AAPH, ABTS, and FRAP. The thirteen screened antioxidants can be identified by Q-TOF-MS/MS. kaempferol-3-O-rutinoside, narcissoside, and astragalin were first reported in GCP. respectively. The thirteen screened antioxidants were quantified simultaneously at their maximum absorption wavelengths, with concentrations ranging from 0.61 to 79.30 mg/g across three distinct flowering stages. Furthermore, the in vitro activity and molecular docking of oxidative stress-related enzyme xanthine oxidase (XOD) were utilized to evaluate the antioxidant capacity of the screened compounds. Consequently, our method effectively screens for antioxidants within complex mixtures, positioning GCP as a promising reservoir of natural bioactive compounds.
一种联合筛选方法的新应用，该方法将AAPH、ABTS和FRAP分析与高效液相色谱（HPLC）相结合，首次用于筛选青梅（GCP）中的天然抗氧化剂。该方法假定，在与AAPH、ABTS和FRAP孵育后，HPLC色谱图中具有抗氧化活性的化合物的峰面积会显著减少或消失。通过Q-TOF-MS/MS可以鉴定出13种筛选出的抗氧化剂。山柰酚-3-O-芸香糖苷、水仙苷和紫云英苷首次分别在青梅中被报道。在三个不同的开花阶段，在其最大吸收波长下同时对13种筛选出的抗氧化剂进行定量，浓度范围为0.61至79.30 mg/g。此外，利用氧化应激相关酶黄嘌呤氧化酶（XOD）的体外活性和分子对接来评估筛选出的化合物的抗氧化能力。因此，我们的方法有效地筛选了复杂混合物中的抗氧化剂，使青梅成为天然生物活性化合物的一个有前景的来源。
