Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM and Engineering & Technology Research Center for Chinese Materia Medica Quality of Guangdong Province, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
Food Chem. 2023 Feb 1;401:134091. doi: 10.1016/j.foodchem.2022.134091. Epub 2022 Sep 5.
A new AAPH-Incubating HPLC-DAD-HR MS/MS method was developed for the rapid and high-throughput screening of antioxidants directly in natural products and applied to Gardenia jasminoides fruit. This method was assumed that the peak areas of compounds with potential antioxidant activity in HPLC chromatograms would be significantly reduced or disappeared after incubating with the AAPH which can release ROO at physiological conditions (37 °C, pH 7.4). Additionally, the activity of antioxidants can be evaluated by comparing the peak reduction rates and the screened components can be further identified by HRMS/MS. Then, 17 potential natural antioxidants from the crude extract of GJF was screened. Among them, three major components including crocin I, crocin II and crocetin showed excellent ROO scavenging activity, which were further validated by the ORAC assay. In conclusion, our study provided a simple and effective strategy to rapidly screen antioxidants in natural products.
建立了一种新的 AAPH-孵育 HPLC-DAD-HR MS/MS 方法，用于快速高通量筛选天然产物中的抗氧化剂，并将其应用于栀子果实。该方法假设在生理条件（37°C，pH 7.4）下，AAPH 可以释放 ROO，具有潜在抗氧化活性的化合物在 HPLC 色谱图中的峰面积会显著降低或消失。此外，还可以通过比较峰降低率来评估抗氧化剂的活性，并且可以通过 HRMS/MS 进一步鉴定筛选的成分。然后，从 GJF 的粗提物中筛选出 17 种潜在的天然抗氧化剂。其中，三种主要成分包括藏红花酸 I、藏红花酸 II 和西红花酸，表现出优异的 ROO 清除活性，这通过 ORAC 测定进一步得到验证。总之，本研究提供了一种简单有效的策略，可快速筛选天然产物中的抗氧化剂。
