Ahmadpour Yasaman, Bahrami Gholamreza, Arkan Elham, Abbaszadeh Fatemeh, Aghaz Faranak, Fakhri Sajad, Echeverría Javier
Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Front Pharmacol. 2025 Feb 14;16:1533025. doi: 10.3389/fphar.2025.1533025. eCollection 2025.
Spinal cord injury (SCI) is a leading cause of sensorimotor disorders, impacting millions of people globally. The absence of effective treatments and the side effects of existing medications highlight the need for innovative research into new therapeutic compounds.
Given the critical role of oxidative stress in the development of SCI and the antioxidant properties of oligosaccharides in other neurological disorders, this study focuses on the role of oxidative stress in SCI and explores the potential of a novel oligosaccharide nanoformulation derived from (Oligo-L).
Oligo-L was formulated using soy lecithin as the phospholipid and the characterization included size, zeta potential, morphology, and drug loading efficiency. Then 35 Wistar male rats were divided into five groups of Sham, SCI, and Oligo-L (10 μL intrathecal injection of 15, 30, and 45 mg/mL). An aneurysm clip was used to induce compression injury of the SCI and Oligo-L groups. Sensory-motor functions were evaluated weekly for 4 weeks using tests such as the BBB scale, inclined plane, acetone drop, hot plate, von Frey, and monitoring of weight changes. Additionally, oxidative stress markers and histological changes were examined to evaluate changes in nitrite, glutathione, catalase, and neuronal survival.
The findings indicated that Oligo-L treatment led to significant improvements in neuropathic pain, and motor function performance and weight of the animals from the first week post-SCI. Oligo-L also enhanced catalase and glutathione levels while reducing serum nitrite levels, contributing to neuronal preservation. Additionally, Oligo-L increased neuronal survival in the both ventral (motor neurons) and dorsal (sensory neurons) horns of the spinal cord.
Overall, Oligo-L, characterized by its beneficial physicochemical properties, showed promising potential as a neuroprotective agent and facilitated the recovery of sensory and motor functions after SCI.
脊髓损伤（SCI）是导致感觉运动障碍的主要原因，全球数百万人受其影响。缺乏有效的治疗方法以及现有药物的副作用凸显了对新型治疗化合物进行创新性研究的必要性。
鉴于氧化应激在脊髓损伤发展过程中的关键作用以及寡糖在其他神经系统疾病中的抗氧化特性，本研究聚焦于氧化应激在脊髓损伤中的作用，并探索一种源自（寡糖-L）的新型寡糖纳米制剂的潜力。
以大豆卵磷脂作为磷脂来制备寡糖-L，其特性表征包括粒径、zeta电位、形态以及载药效率。然后将35只雄性Wistar大鼠分为假手术组、脊髓损伤组和寡糖-L组（鞘内注射10μL，浓度分别为15、30和45mg/mL）。使用动脉瘤夹对脊髓损伤组和寡糖-L组诱导压迫性损伤。在4周内每周使用如BBB量表、倾斜平面、丙酮滴注、热板、von Frey等测试以及监测体重变化来评估感觉运动功能。此外，检测氧化应激标志物和组织学变化，以评估亚硝酸盐、谷胱甘肽、过氧化氢酶和神经元存活情况的变化。
研究结果表明，寡糖-L治疗可使脊髓损伤后第一周动物的神经性疼痛、运动功能表现和体重得到显著改善。寡糖-L还提高了过氧化氢酶和谷胱甘肽水平，同时降低了血清亚硝酸盐水平，有助于神经元的保存。此外，寡糖-L增加了脊髓腹角（运动神经元）和背角（感觉神经元）的神经元存活。
总体而言，具有有益理化性质的寡糖-L作为一种神经保护剂显示出有前景的潜力，并促进了脊髓损伤后感觉和运动功能的恢复。
