Jonker M A, van Roon P S, den Hartog J M
Z Lebensm Unters Forsch. 1985 Mar;180(3):202-6. doi: 10.1007/BF01027265.
Quality regulations of the Guelders ring sausage are applied in the Netherlands. One of the quality parameters is the content of CFMP (collagen-free muscle protein) in the product. The purpose of this research is to substitute the usual indirect method by a direct analytical method for CFMP. The proposed direct method is composed of separation and determination of actin. Actin was well separated from the sausage product by SDS-gel-filtration chromatography, which was checked by SDS-polyacrylamide-gel electrophoresis. Actin was determined by its 3-methylhistidine content, but this result was smaller than expected. It was caused by the presence of salt during the 3-methylhistidine analysis. Finally, a method for the determination of actin by 3-methylhistidine was developed for the direct procedure. The relations of 3 MH (3-methylhistidine) with respective CFMP and MP (muscle protein) are formulated. Both formulae give a reasonably good estimation of 3 MH, CFMP or MP, one of these parameters of the Guelders ring sausage being known.
荷兰应用了聚圈香肠的质量规定。其中一个质量参数是产品中无胶原蛋白肌肉蛋白（CFMP）的含量。本研究的目的是用一种直接分析方法替代通常的间接方法来测定CFMP。所提出的直接方法包括肌动蛋白的分离和测定。通过SDS - 凝胶过滤色谱法可将肌动蛋白从香肠产品中很好地分离出来，这通过SDS - 聚丙烯酰胺凝胶电泳进行了验证。通过肌动蛋白的3 - 甲基组氨酸含量来测定肌动蛋白，但该结果比预期的要小。这是由于在3 - 甲基组氨酸分析过程中存在盐分。最后，为直接测定法开发了一种通过3 - 甲基组氨酸测定肌动蛋白的方法。给出了3 - MH（3 - 甲基组氨酸）与相应的CFMP和MP（肌肉蛋白）之间的关系公式。已知聚圈香肠的这些参数之一时，这两个公式都能对3 - MH、CFMP或MP给出相当不错的估算。
