Jonker M A, van Roon P S, den Hartog J M
Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Z Lebensm Unters Forsch. 1987 Nov;185(5):402-5. doi: 10.1007/BF01042263.
The purpose of this study was to find experimental conditions for the complete solubility of collagen-free muscle proteins (CFMP) using acetone powder of Guelders ring sausage. Preliminary experiments were carried out to choose the best procedure for preparing the acetone dry powder. Two different methods of acetone extraction of minced sausage were compared. The acetone dry mass (ADM) method using continuous extraction in a Soxhlet [2] apparatus gave better results than the acetone powder (ACP) method, which used a blender [1]. The ADM method was used for further investigations. ADM was extracted with two types of sodium dodecyl sulphate (SDS), containing solvents A and B. Solvent A contains a Tris-boric acid buffer (pH 8.2) with 1.5% (m/v) SDS and 0.05% (m/v) dithioerythreitol [3]. Solvent B is a borate-chloric acid buffer (pH 9.0) with 2.0% (m/v) SDS and 1.0% (m/v) mercapto-ethanol [2]. Both solvents showed a linear relationship between the quantities of CFMP in ADM and the dissolved CFMP. The linear relationships were found between quantities of 10.0 and 30.0 mg (solution A) and of 5.0 and 30.0 mg ADM (solution B) per ml solvent. The solubility of CFMP was better in solvent B than in solution A. Completely dissolved CFMP from ADM was only obtained in the case of 5.0 mg ADM in 1.0 ml solution B. These conditions will be used in liquid chromatography experiments, the results of which will be reported later.
本研究的目的是利用荷兰风笛肠的丙酮粉找到使无胶原蛋白肌肉蛋白（CFMP）完全溶解的实验条件。进行了初步实验以选择制备丙酮干粉的最佳程序。比较了两种不同的丙酮提取碎肉肠的方法。使用索氏提取器[2]连续提取的丙酮干质量（ADM）法比使用搅拌机的丙酮粉（ACP）法[1]效果更好。ADM法用于进一步研究。用两种含有溶剂A和B的十二烷基硫酸钠（SDS）提取ADM。溶剂A含有Tris-硼酸缓冲液（pH 8.2），其中含有1.5%（m/v）的SDS和0.05%（m/v）的二硫代赤藓糖醇[3]。溶剂B是一种硼酸盐-氯酸缓冲液（pH 9.0），含有2.0%（m/v）的SDS和1.0%（m/v）的巯基乙醇[2]。两种溶剂在ADM中的CFMP量与溶解的CFMP之间均呈现线性关系。在每毫升溶剂中，10.0至30.0毫克（溶液A）以及5.0至30.0毫克ADM（溶液B）的量之间发现了线性关系。CFMP在溶剂B中的溶解度比在溶液A中更好。仅在1.0毫升溶液B中含有5.0毫克ADM的情况下才能从ADM中获得完全溶解的CFMP。这些条件将用于液相色谱实验，其结果将在稍后报告。
