Miteva E
Vet Med Nauki. 1984;21(7-8):94-9.
Stated are the conditions under which the extraction of lipids and their quantitative evaluation by classes in meat products could be carried out through the combined use of thin-layer and gas chromatography. The amounts of neutral class lipids were determined by the high-molecular fatty acids contained in them in the form of methyl esters. Their re-esterification was performed directly on silica gel with methanol and BF3 catalyst in a strongly alkaline medium in airtight flasks. There was methylation not only of the free fatty acids, but also of the bound ones thanks to the processes of saponification and esterification which ran their course simultaneously. This procedure shortened the time for analysis and made analytical work more feasible. Differences were found in the content of triglycerides and free fatty acids between Rousse ham, Hemous alami, and Turnovska loukanka (a highly seasoned sausage) that were due to he various technologic regimes of production, at a standard error of the mean values of from 0.11 to 1.11.
阐述了通过薄层色谱和气相色谱联用,在肉制品中进行脂质提取及其按类别进行定量评估的条件。中性类脂质的含量通过其中以甲酯形式存在的高分子脂肪酸来测定。它们的重新酯化直接在硅胶上,于密闭烧瓶中在强碱性介质中用甲醇和BF₃催化剂进行。由于皂化和酯化过程同时进行,不仅游离脂肪酸发生了甲基化,结合脂肪酸也发生了甲基化。该程序缩短了分析时间,使分析工作更可行。在鲁塞火腿、赫穆斯阿拉米和图尔诺夫斯卡卢坎卡(一种调味浓郁的香肠)之间,发现甘油三酯和游离脂肪酸含量存在差异,这是由于不同的生产工艺制度导致的,平均值的标准误差为0.11至1.11。