Priadi Gunawan, Octaviana Senlie, Pramono Ajeng Kusumaningtyas, Sari Titi Dwi, Afiati Fifi, Setiyoningrum Fitri, Wibowo Des Saputro, Meliah Siti, Nofiani Risa
Research Center for Applied Microbiology, National Research and Innovation Agency, Bogor, Indonesia.
Research Center for Biomedical, National Research and Innovation Agency, Bogor, Indonesia.
Curr Microbiol. 2025 Mar 3;82(4):164. doi: 10.1007/s00284-025-04123-2.
Myxobacteria are well known for producing secondary metabolites with various potential applications, including volatile compounds that serve as flavors, although this capability is less documented. This study characterizes the morphology of six Myxococcus strains (InaCC B1497, B1482, B1498, B1483, B1484, and B1486) obtained from the Indonesian Culture Collection (InaCC) and evaluates their potential for flavor biotransformation. Morphological observations were conducted on fruiting bodies, vegetative cells, myxospores, and colony shapes using dissection and binocular microscopes. All strains were identified as Myxococcus with 99% similarity to Myxococcus stipitatus DSM14675T based on 16S rRNA gene sequencing. Strains InaCC B1484 and B1486 demonstrated the ability to convert geraniol into various terpenes and hydrocarbons, with strain B1486 producing high levels of citral and geranyl oleate, while B1484 yielded significant amounts of geranial and nerol. Both isolates also showed capability for limonene conversion, with B1486 displaying a broader range of aromatic hydrocarbons. Draft genome sequences for strains InaCC B1484 and B1486, each approximately 10.75 Mbp, revealed 99.99% ANI (average nucleotide identity) with each other and 89.5% ANI with the reference genome Myxococcus stipitatus DSM14675T. Both genomes contain genes associated with terpenoid and polyketide biosynthesis, including pathways for geraniol and limonene degradation.
粘细菌以产生具有各种潜在应用的次生代谢产物而闻名，包括用作香料的挥发性化合物，尽管这种能力的文献记载较少。本研究对从印度尼西亚菌种保藏中心（InaCC）获得的六种粘球菌菌株（InaCC B1497、B1482、B1498、B1483、B1484和B1486）的形态进行了表征，并评估了它们进行风味生物转化的潜力。使用解剖显微镜和双目显微镜对子实体、营养细胞、粘孢子和菌落形态进行了观察。基于16S rRNA基因测序，所有菌株均被鉴定为粘球菌，与茎生粘球菌DSM14675T的相似性为99%。InaCC B1484和B1486菌株表现出将香叶醇转化为各种萜类化合物和碳氢化合物的能力，B1486菌株产生高水平的柠檬醛和香叶基油酸酯，而B1484菌株产生大量的香叶醛和橙花醇。这两种分离株还表现出转化柠檬烯的能力，B1486菌株显示出更广泛的芳香烃范围。InaCC B1484和B1486菌株的基因组草图序列，每个约10.75 Mbp，彼此之间的平均核苷酸同一性（ANI）为99.99%，与参考基因组茎生粘球菌DSM14675T的ANI为89.5%。两个基因组都包含与萜类化合物和聚酮化合物生物合成相关的基因，包括香叶醇和柠檬烯降解途径。
