一种多相方法鉴定出了纤维素分解菌属堆囊菌属的七个新物种，即新种堆囊菌酸腐堆囊菌、新种沙质堆囊菌、新种保加利亚堆囊菌、新种达维德堆囊菌、新种肯尼亚堆囊菌、新种东方堆囊菌和新种赖兴巴赫堆囊菌。
A polyphasic approach leads to seven new species of the cellulose-decomposing genus Sorangium, Sorangium ambruticinum sp. nov., Sorangium arenae sp. nov., Sorangium bulgaricum sp. nov., Sorangium dawidii sp. nov., Sorangium kenyense sp. nov., Sorangium orientale sp. nov. and Sorangium reichenbachii sp. nov.
作者信息
Mohr Kathrin I, Wolf Corinna, Nübel Ulrich, Szafrańska Anna K, Steglich Matthias, Hennessen Fabienne, Gemperlein Katja, Kämpfer Peter, Martin Karin, Müller Rolf, Wink Joachim
机构信息
1Microbial Drugs, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany.
2Microbial Strain Collection, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany.
出版信息
Int J Syst Evol Microbiol. 2018 Nov;68(11):3576-3586. doi: 10.1099/ijsem.0.003034. Epub 2018 Sep 20.
Seventy-three strains of Sorangium have been isolated from soil samples collected from all over the world. The strains were characterized using a polyphasic approach and phenotypic, genotypic and chemotype analyses clarified their taxonomic relationships. 16S rRNA, xynB1, groEL1, matrix-assisted laser desorption/ioniziation time-of-flight mass spectrometry and API-ZYM analyses were conducted. In addition, from selected representative strains, fatty acids, quinones and phospholipids were analysed. In silico DNA-DNA hybridization and DNA-DNA hybridization against the current type species of Sorangiumcellulosum strain Soce 1871 (DSM 14627) completed the analyses. Finally, our study revealed seven new species of Sorangium: Sorangium ambruticinum (Soce 176; DSM 53252, NCCB 100639, sequence accession number ERS2488998), Sorangium arenae (Soce 1078; DSM 105768, NCCB 100643, ERS2489002), Sorangium bulgaricum (Soce 321; DSM 53339, NCCB 100640, ERS2488999), Sorangium dawidii (Soce 362; DSM 105767, NCCB 100641, ERS2489000), Sorangium kenyense (Soce 375; DSM 105741, NCCB 100642, ERS2489001), Sorangium orientale (Soce GT47; DSM 105742, NCCB 100638, ERS2501484) and Sorangium reichenbachii (Soce 1828; DSM 105769, NCCB 100644, ERS2489003).
已从世界各地采集的土壤样本中分离出73株堆囊菌属菌株。采用多相分类方法对这些菌株进行了表征，表型、基因型和化学型分析明确了它们的分类关系。进行了16S rRNA、木聚糖酶基因xynB1、分子伴侣蛋白groEL1、基质辅助激光解吸/电离飞行时间质谱分析和API-ZYM分析。此外，还对选定的代表性菌株进行了脂肪酸、醌类和磷脂分析。通过计算机模拟DNA-DNA杂交以及与纤维素堆囊菌菌株Soce 1871（DSM 14627）的现行模式种进行DNA-DNA杂交，完成了分析。最后，我们的研究揭示了7个新的堆囊菌属物种：粗糙堆囊菌（Soce 176；DSM 53252，NCCB 100639，序列登录号ERS2488998）、沙地堆囊菌（Soce 1078；DSM 105768，NCCB 100643，ERS2489002）、保加利亚堆囊菌（Soce 321；DSM 53339，NCCB 100640，ERS2488999）、大卫堆囊菌（Soce 362；DSM 105767，NCCB 100641，ERS2489000）、肯尼亚堆囊菌（Soce 375；DSM 105741，NCCB 100642，ERS2489001）、东方堆囊菌（Soce GT47；DSM 105742，NCCB 100638，ERS2501484）和赖兴巴赫堆囊菌（Soce 1828；DSM 105769，NCCB 100644，ERS2489003）。
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