Fudou Ryosuke, Jojima Yasuko, Iizuka Takashi, Yamanaka Shigeru
Laboratory of General and Applied Microbiology, Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture,Setagaya-ku, Tokyo 156-8502, Japan.
J Gen Appl Microbiol. 2002 Apr;48(2):109-16. doi: 10.2323/jgam.48.109.
Phenotypic and phylogenetic studies were performed on two myxobacterial strains, SMP-2 and SMP-10, isolated from coastal regions. The two strains are morphologically similar, in that both produce yellow fruiting bodies, comprising several sessile sporangioles in dense packs. They are differentiated from known terrestrial myxobacteria on the basis of salt requirements (2-3% NaCl) and the presence of anteiso-branched fatty acids. Comparative 16S rRNA gene sequencing studies revealed that SMP-2 and SMP-10 are genetically related, and constitute a new cluster within the myxobacteria group, together with the Polyangium vitellinum Pl vt1 strain as the closest neighbor. The sequence similarity between the two strains is 95.6%. Based on phenotypic and phylogenetic evidence, it is proposed that these two strains be assigned to a new genus, Haliangium gen. nov., with SMP-2 designated as Haliangium ochraceum sp. nov. (= JCM 11303(T) = DSM 14365(T)), and SMP-10 as Haliangium tepidum sp. nov. (= JCM 11304(T)= DSM 14436(T)).
对从沿海地区分离出的两株粘细菌菌株SMP - 2和SMP - 10进行了表型和系统发育研究。这两株菌株在形态上相似，都产生黄色子实体，由密集排列的几个无柄小孢子囊组成。根据盐需求（2 - 3% NaCl）和反异分支脂肪酸的存在，它们与已知的陆生粘细菌有所区别。比较16S rRNA基因测序研究表明，SMP - 2和SMP - 10在遗传上相关，与最接近的邻居黄柄游动放线菌Pl vt1菌株一起，在粘细菌群内构成一个新的簇。两株菌株之间的序列相似性为95.6%。基于表型和系统发育证据，建议将这两株菌株归入一个新属，即海栖粘细菌属（Haliangium gen. nov.），其中SMP - 2被指定为赭色海栖粘细菌（Haliangium ochraceum sp. nov.）（= JCM 11303(T) = DSM 14365(T)），SMP - 10被指定为温热海栖粘细菌（Haliangium tepidum sp. nov.）（= JCM 11304(T)= DSM 14436(T)）。
