Liang Shaochan, Xu Siyuan, Ye ShengLian, Liang Lang, Li Hongliang, Liu Jiaye, Zhang Yao, Zou Feng, Liang Xiaodan, Tan Bo, Cao Hongying
School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510378, China.
J Agric Food Chem. 2025 Mar 12;73(10):5970-5980. doi: 10.1021/acs.jafc.4c11577. Epub 2025 Mar 3.
Neuromuscular conduction dysfunction-induced underactive bladder (UAB) is a major urological complication associated with diabetes mellitus (DM), and there remain deficiencies in reliable pharmacological treatment options. Astragalus polysaccharides (APS), as an edible active substance in , have a therapeutic effect on diabetes and its complications. We investigated the effects and mechanism of APS in high-fat-diet-induced (HFD) diabetic UAB mice. APS significantly reduced fasting plasma glucose, insulin, and HOMA-IR index. Furthermore, APS treatment significantly decreased maximum bladder capacity, residual volume, bladder compliance, contraction intervals, empty and full resting pressure, and increased voiding volume and voided efficiency. In addition, APS ameliorated the hyporesponsiveness of purinergic and cholinergic-mediated neuromuscular contraction of the detrusor and improved the dysregulation of inhibitory and excitatory neurotransmission by downregulating the levels of nNOS and VIP, and upregulating ChAT and SP in HFD mice. This study revealed that APS ameliorates diabetic UAB via the normalization of neuromuscular conduction.
神经肌肉传导功能障碍所致的膀胱活动低下（UAB）是糖尿病（DM）相关的主要泌尿系统并发症，可靠的药物治疗方案仍存在不足。黄芪多糖（APS）作为一种可食用的活性物质，对糖尿病及其并发症具有治疗作用。我们研究了APS对高脂饮食诱导（HFD）的糖尿病UAB小鼠的影响及其机制。APS显著降低了空腹血糖、胰岛素和HOMA-IR指数。此外，APS治疗显著降低了最大膀胱容量、残余尿量、膀胱顺应性、收缩间隔、排空和充盈静息压力，并增加了排尿量和排尿效率。此外，APS改善了HFD小鼠中嘌呤能和胆碱能介导的逼尿肌神经肌肉收缩反应低下的情况，并通过下调nNOS和VIP水平以及上调ChAT和SP水平改善了抑制性和兴奋性神经传递的失调。本研究表明，APS通过使神经肌肉传导正常化来改善糖尿病性UAB。
