Savchak Oksana K, Gumuscu Burcu
Biosensors and Devices Lab, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, Netherlands.
Institute of Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, Netherlands.
Lab Chip. 2025 Mar 25;25(7):1776-1786. doi: 10.1039/d4lc00947a.
We introduce a robust multilayer dielectric stack for digital microfluidic chips to withstand the humid conditions of cell culture incubators for at least 60 days. Consisting of a combination of 1 μm polyvinylidene difluoride and 5 μm SU-8 layers, the stack demonstrated high breakdown voltages up to 1600 V and minimal surface currents <30 nA at 100 V. Long-term stability and precision in liquid handling enabled us to study macrophage phenotype modulation, pro-inflammatory response induction in macrophage population with single cell cytokine quantification and testing of a potentially anti-inflammatory drug candidate TCB-2 and its influence on macrophage phenotype, morphology, and cytokine release. The multilayer dielectric stack offers a durable solution for long-term biological assays on digital microfluidic platforms.
我们为数字微流控芯片引入了一种坚固的多层介电堆栈，使其能够在细胞培养箱的潮湿环境中耐受至少60天。该堆栈由1μm聚偏二氟乙烯和5μm SU-8层组合而成，在100V时表现出高达1600V的高击穿电压和小于30nA的最小表面电流。液体处理中的长期稳定性和精度使我们能够研究巨噬细胞表型调节、通过单细胞细胞因子定量在巨噬细胞群体中诱导促炎反应，以及测试一种潜在的抗炎药物候选物TCB-2及其对巨噬细胞表型、形态和细胞因子释放的影响。多层介电堆栈为数字微流控平台上的长期生物学检测提供了一种耐用的解决方案。
