Jiang Fengze, Zhang Cui, Wang Xiangnan, Yin Yuan, Hong Jieling, Tang Hao, Tang Li-Juan, Wu Zhenkun
State Key Laboratory of Chemo/Bio-Sensing, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
School of Resource & Environment, Hunan University of Technology and Business, Changsha, Hunan 410082, China.
Analyst. 2025 Mar 24;150(7):1229-1234. doi: 10.1039/d5an00099h.
We report a novel DNA lesion-modified nanotweezer that underwent conformational switching upon ALKBH enzyme-mediated lesion repair. We demonstrated that the DNA lesion-modified nanotweezer enabled specific and rapid monitoring of ALKBH2 and ALKBH3 activities and screening of inhibitors. Importantly, this strategy allowed evaluating ALKBH2-associated drug resistance in cells.
我们报道了一种新型的DNA损伤修饰纳米镊子，它在ALKBH酶介导的损伤修复过程中发生构象转换。我们证明，这种DNA损伤修饰纳米镊子能够特异性且快速地监测ALKBH2和ALKBH3的活性，并筛选抑制剂。重要的是，该策略能够评估细胞中与ALKBH2相关的耐药性。
